RETRACTED: Production of viable chicken by allogeneic transplantation of primordial germ cells induced from somatic cells (Retracted article. See vol. 14, 2023)

被引：21

作者：

Zhao, Ruifeng ^{[1
,2
]}

Zuo, Qisheng ^{[1
,2
]}

Yuan, Xia ^{[1
,2
]}

Jin, Kai ^{[1
,2
]}

Jin, Jing ^{[1
,2
]}

Ding, Ying ^{[1
,2
]}

Zhang, Chen ^{[1
,2
]}

Li, Tingting ^{[1
,2
]}

Jiang, Jingyi ^{[1
,2
]}

Li, Jiancheng ^{[1
,2
]}

Zhang, Ming ^{[1
,2
]}

Shi, Xiang ^{[1
,2
]}

Sun, Hongyan ^{[1
,2
]}

Zhang, Yani ^{[1
,2
]}

Xu, Qi ^{[1
,2
]}

Chang, Guobin ^{[1
,2
]}

Zhao, Zhenhua ^{[3
]}

Li, Bing ^{[3
]}

Wu, Xinsheng ^{[1
,2
]}

Zhang, Yang ^{[1
,2
]}

Song, Jiuzhou ^{[4
]}

Chen, Guohong ^{[1
,2
]}

Li, Bichun ^{[1
,2
]}

机构：

[1] Yangzhou Univ, Coll Anim Sci & Technol, Key Lab Anim Breeding Reprod & Mol Design Jiangsu, Yangzhou, Jiangsu, Peoples R China

[2] Yangzhou Univ, Minist Educ China, Joint Int Res Lab Agr & Agriprod Safety, Yangzhou, Jiangsu, Peoples R China

[3] Chinese Acad Agr Sci, Poultry Res Inst, Yangzhou, Jiangsu, Peoples R China

[4] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA

来源：

NATURE COMMUNICATIONS | 2021年 / 12卷 / 01期

基金：

中国国家自然科学基金;

关键词：

PLURIPOTENT STEM-CELLS; IN-VITRO; CRUCIAL ROLE; DIFFERENTIATION; PATHWAY; NANOG;

D O I：

10.1038/s41467-021-23242-5

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The allogeneic transplantation of primordial germ cells (PGCs) derived from somatic cells overcomes the limitation of avian cloning. Here, we transdifferentiate chicken embryo fibroblasts (CEFs) from black feathered Langshan chickens to PGCs and transplant them into White Plymouth Rock chicken embryos to produce viable offspring with characteristics inherited from the donor. We express Oct4/Sox2/Nanog/Lin28A (OSNL) to reprogram CEFs to induced pluripotent stem cells (iPSCs), which are further induced to differentiate into PGCs by BMP4/BMP8b/EGF. DNA demethylation, histone acetylation and glycolytic activation elevate the iPSC induction efficiency, while histone acetylation and glycolytic inhibition facilitate PGCs formation. The induced PGCs (iPGCs) are transplanted into the recipients, which are self-crossed to produce 189/509 somatic cells derived chicken with the donor's characteristics. Microsatellite analysis and genome sequencing confirm the inheritance of genetic information from the donor. Thus, we demonstrate the feasibility of avian cloning from somatic cells.

引用

页数：13

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