Amphotericin B resistance leads to enhanced proteinase and phospholipase activity and reduced germ tube formation in Candida albicans

被引：26

作者：

Kumar, Rohitashw ^{[1
]}

Shukla, P. K. ^{[1
]}

机构：

[1] CSIR, Cent Drug Res Inst, Div Fermentat Technol, Med Mycol Lab, Lucknow 226001, Uttar Pradesh, India

来源：

FUNGAL BIOLOGY | 2010年 / 114卷 / 2-3期

关键词：

Amphotericin B; Antifungal resistance; Candida albicans; Virulence factors; ANTIFUNGAL ACTIVITY; FLUCONAZOLE RESISTANCE; IN-VITRO; POLYENE; YEASTS; GROWTH; GENES; ASSAY;

D O I：

10.1016/j.funbio.2009.12.003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Resistance to amphotericin B is an emerging phenomenon in Candida albicans. Amphotericin B-resistant strain of C. albicans was developed under laboratory conditions and the stability of acquired resistance was confirmed in vitro as well as in vivo. This AMB-resistant strain exhibited reduced germ tube formation as compared to parent strain of C. albicans (ATCC10231). Enzymatic activity of virulence factors like secreted aspartyl proteinase and phospholipase were found to be significantly high in AMB-R as compared to parent strain whereas ergosterol content of AMB-R was drastically reduced. The behavior of AMB-R strain is an interesting phenomenon and opens up a wide area of research regarding pathways and mechanisms. (C) 2009 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.

引用

页码：189 / 197

页数：9

共 30 条

[1] A RAPID COLORIMETRIC ASSAY FOR DETERMINATION OF LEUKOCYTE-MEDIATED INHIBITION OF MYCELIAL GROWTH OF CANDIDA-ALBICANS [J].