Carbon nanotube composite as novel platform for microbial biosensor

被引：25

作者：

Anik, Ulku ^{[1
]}

Timur, Suna

Odaci, Dilek

Perez, Briza

Alegret, Salvador

Merkoci, Arben

机构：

[1] Mugla Univ, Fac Art & Sci, Dept Chem, TR-4800 Kotekli, Mugla, Turkey

[2] Ege Univ, Fac Sci, Dept Biochem, TR-35100 Izmir, Turkey

[3] Univ Autonoma Barcelona, Dept Quim, Grp Sensors & Biosensors, Bellaterra 08193, Catalonia, Spain

[4] Univ Autonoma Barcelona, Inst Catala Nanotecnol, Bellaterra 08193, Catalonia, Spain

来源：

ELECTROANALYSIS | 2007年 / 19卷 / 7-8期

关键词：

microbial biosensor; Pseudomonas fluoresccns; carbon nanotube; graphite-epoxy composite;

D O I：

10.1002/elan.200603786

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The presented work includes the development of a microbial biosensor based on a carbon-nanotube epoxy composite (CNTEC) platform used as supporting electrode for cell immobilization. For this purpose, cells of Pseudomonas fluorescens were immobilized on the surface of the CNTEC electrode by means of gelatin which was then cross linked with glutaraldehyde. After optimization of experimental parameters like cell amount, pH and temperature, the system was calibrated for glucose. From the calibration graph the linear range was estimated as 0.5 - 4.0 mM with a response time of 100 s. Furthermore, substrate specificity and operational stability were investigated. Finally, the results that were obtained with CNTEC electrodes were compared with conventional graphite epoxy composite electrode (GECE) and as a result, higher current values (2 to 3 folds) were observed with CNTEC microbial biosensor.

引用

页码：893 / 898

页数：6

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