Preparation of Carbon Dots for Cellular Imaging by the Molecular Aggregation of Cellulolytic Enzyme Lignin

被引：89

作者：

Niu, Na ^{[1
,2
]}

Ma, Zhuoming ^{[1
]}

He, Fei ^{[3
]}

Li, Shujun ^{[1
]}

Li, Jian ^{[1
]}

Liu, Shouxin ^{[1
]}

Yang, Piaoping ^{[3
]}

机构：

[1] Northeast Forestry Univ, Minist Educ, Key Lab Biobased Mat Sci & Technol, Harbin 150040, Peoples R China

[2] Northeast Forestry Univ, Coll Sci, Harbin 150040, Peoples R China

[3] Harbin Engn Univ, Coll Mat Sci & Chem Engn, Minist Educ, Key Lab Superlight Mat & Surface Technol, Harbin 150001, Peoples R China

来源：

LANGMUIR | 2017年 / 33卷 / 23期

基金：

中国博士后科学基金;

关键词：

SEMICONDUCTOR QUANTUM DOTS; ONE-STEP SYNTHESIS; HIGHLY LUMINESCENT; HYDROTHERMAL CARBONIZATION; ACTIVATED CARBON; GREEN SYNTHESIS; NANODOTS; NANOPARTICLES; CELLS; NANOSTRUCTURES;

D O I：

10.1021/acs.langmuir.7b00617

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Carbon dots, which are less than 10 nm in diameter, have been widely investigated because of their unique luminescence properties and potential for use in bioimaging. In the present work, natural carbon dots (L-CDs) were obtained by molecular aggregation, using ethanol-extracted cellulolytic enzyme lignin. The whole process for the preparation of L-CDs was green and simple to operate and did not use toxic chemical reagents or harsh conditions. The newly prepared L-CDs emitted multicolor photoluminescence following one- and two-photon excitation. The L-CDs also showed good cellular biocompatibility, which is crucial for biological applications. One- and two-photon cell-imaging studies demonstrated the potential of L-CDs for bioimaging.

引用

页码：5786 / 5795

页数：10

共 53 条

[21] Effect of non-enzymatic proteins on enzymatic hydrolysis and simultaneous saccharification and fermentation of different lignocellulosic materials [J].