Effects of sugars and growth regulators on in vitro growth of Dactylorhiza species

被引:30
|
作者
Wotavova-Novotna, K. [1 ]
Vejsadova, H.
Kindlmann, P.
机构
[1] Acad Sci Czech Republ, Inst Syst Biol & Ecol, CZ-37005 Ceske Budejovice, Czech Republic
[2] Silva Tarouca Res Inst Landscape & Ornamental Gar, CZ-25243 Pruhonice, Czech Republic
[3] Univ S Bohemia, Fac Agr, CZ-37005 Ceske Budejovice, Czech Republic
关键词
auxins; cytokinins; glucose; in vitro cultivation; sucrose; terrestrial orchids;
D O I
10.1007/s10535-007-0040-x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The influence of sugars and growth regulators on shoot and root growth of Dactylorhiza species was studied under in vitro conditions. The seedling development was stimulated with the application of glucose and sucrose at concentration of 10 g dm(-3) each. The improvement of shoot growth rate and shoot length was enhanced by cytokinins N-6-(2-isopentenyl)adenine or N-6-benzyladenine and their combination with auxin indolebutyric acid (IBA). The root growth rate and root length of seedlings increased in the presence of IBA and a-naphthaleneacetic acid. Individual Dactylorhiza species showed statistically significant differences in shoot and root development depending on sugar and growth regulator combinations.
引用
收藏
页码:198 / 200
页数:3
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