Kinetics of Salmonella typhimurium binding with antibody by immunoassays using a surface plasmon resonance biosensor

被引:4
作者
Chung, Kyong-Hwan [1 ,2 ]
Hwang, Hyun-Soo [4 ]
Lee, Ki-Young [1 ,3 ]
机构
[1] Chonnam Natl Univ, Dept Appl Chem Engn, Kwangju 500757, South Korea
[2] Chonnam Natl Univ, Ctr Funct Nano Fine Chem, Kwangju 500757, South Korea
[3] Chonnam Natl Univ, Res Inst Catalysis, Kwangju 500757, South Korea
[4] Chonnam Natl Univ, Dept Mat & Biochem Engn, Kwangju 500757, South Korea
关键词
Kinetics; Immunoassays; Salmonella typhimurium; Surface plasmon resonance; Antibody; MONOCLONAL-ANTIBODY; STAPHYLOCOCCUS-AUREUS;
D O I
10.1016/j.jiec.2010.01.009
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
This study examined the kinetics of Salmonella typhimurium binding to an antibody using a direct assay and sandwich assay with a surface plasmon resonance (SPR) biosensor. A kinetic model was proposed based on curve-fitting of the experimental SPR sensorgrams using an integral rate equation. The SPR sensitivity in the sandwich assay was significantly higher than that in the direct assay. The association rate constants and affinities of the cell in the sandwich assay were double those in the direct assay. The enhanced binding attraction between the cell and detection antibody in the sandwich assay improved the lower limit of detection. (C) 2010 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:115 / 118
页数:4
相关论文
共 50 条
[31]   Real-time immunoassay of antibody activity in serum by surface plasmon resonance biosensor [J].
Pei, RJ ;
Cui, XQ ;
Yang, XR ;
Wang, EK .
TALANTA, 2000, 53 (03) :481-488
[32]   Using monoclonal antibody to determine lead ions with a localized surface plasmon resonance fiber-optic biosensor [J].
Lin, Tsao-Jen ;
Chung, Mon-Fu .
SENSORS, 2008, 8 (01) :582-593
[33]   Continuous Detection Method for Ractopamine with Surface Plasmon Resonance Biosensor [J].
Li Hui ;
Cai Hao-Yuan ;
Chen Xing ;
Sun Jian-Hai ;
Zhang Lu-Lu ;
Cui Da-Fu .
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2010, 38 (03) :381-384
[34]   A novel multichannel surface plasmon resonance biosensor [J].
Homola, J ;
Lu, HBB ;
Nenninger, GG ;
Dostálek, J ;
Yee, SS .
SENSORS AND ACTUATORS B-CHEMICAL, 2001, 76 (1-3) :403-410
[35]   Surface plasmon resonance biosensor for microalbumin detection [J].
Liu, Jen-Tsai ;
Lin, Po-Shen ;
Hsin, Yue-Ming ;
Tsai, Jang-Zern ;
Chen, Wen-Yih .
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS, 2011, 42 (05) :696-700
[36]   A Novel Photonic Crystal Fiber Biosensor Using Surface Plasmon Resonance [J].
Rifat, A. A. ;
Mahdiraji, G. Amouzad ;
Shee, Y. G. ;
Shawon, Md. Jubayer ;
Adikan, F. R. Mahamd .
INTERNATIONAL CONFERENCE ON MATERIALS FOR ADVANCED TECHNOLOGIES (ICMAT2015) - 4TH PHOTONICS GLOBAL CONFERENCE 2015, 2016, 140 :1-7
[37]   Fragment Screening Using Surface Plasmon Resonance Optical Biosensor Technology [J].
Miura, Takaaki .
YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 2010, 130 (03) :341-348
[38]   Antibody characterization and immunoassays for palytoxin using an SPR biosensor [J].
Yakes, Betsy Jean ;
DeGrasse, Stacey L. ;
Poli, Mark ;
Deeds, Jonathan R. .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2011, 400 (09) :2865-2869
[39]   Antibody characterization and immunoassays for palytoxin using an SPR biosensor [J].
Betsy Jean Yakes ;
Stacey L. DeGrasse ;
Mark Poli ;
Jonathan R. Deeds .
Analytical and Bioanalytical Chemistry, 2011, 400
[40]   Real-time and rapid detection of Salmonella Typhimurium using an inexpensive lab-built surface plasmon resonance setup [J].
Lukose, Jijo ;
Shetty, Vignesh ;
Ballal, Mamatha ;
Chidangil, Santhosh ;
Sinha, Rajeev K. .
LASER PHYSICS LETTERS, 2018, 15 (07)