Kinetics of Salmonella typhimurium binding with antibody by immunoassays using a surface plasmon resonance biosensor

被引：4

作者：

Chung, Kyong-Hwan ^{[1
,2
]}

Hwang, Hyun-Soo ^{[4
]}

Lee, Ki-Young ^{[1
,3
]}

机构：

[1] Chonnam Natl Univ, Dept Appl Chem Engn, Kwangju 500757, South Korea

[2] Chonnam Natl Univ, Ctr Funct Nano Fine Chem, Kwangju 500757, South Korea

[3] Chonnam Natl Univ, Res Inst Catalysis, Kwangju 500757, South Korea

[4] Chonnam Natl Univ, Dept Mat & Biochem Engn, Kwangju 500757, South Korea

来源：

JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY | 2010年 / 16卷 / 01期

关键词：

Kinetics; Immunoassays; Salmonella typhimurium; Surface plasmon resonance; Antibody; MONOCLONAL-ANTIBODY; STAPHYLOCOCCUS-AUREUS;

D O I：

10.1016/j.jiec.2010.01.009

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This study examined the kinetics of Salmonella typhimurium binding to an antibody using a direct assay and sandwich assay with a surface plasmon resonance (SPR) biosensor. A kinetic model was proposed based on curve-fitting of the experimental SPR sensorgrams using an integral rate equation. The SPR sensitivity in the sandwich assay was significantly higher than that in the direct assay. The association rate constants and affinities of the cell in the sandwich assay were double those in the direct assay. The enhanced binding attraction between the cell and detection antibody in the sandwich assay improved the lower limit of detection. (C) 2010 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.

引用

页码：115 / 118

页数：4

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