Proliferation of CD8+ in culture of human T cells derived from peripheral blood of adult donors and cord blood of newborns

As during replicative senescence either in vivo or in vitro, the growing up subpopulation of CD8+CD28- cells is observed, we compared replicative senescence of T cells derived from mononuclear cells of peripheral blood (PBMC) of adults with those from cord blood (CBMC), not having yet CD8+CD28- subpopulation. In PHA-stimulated and IL-2-dependent cultures, T-cells from both cord blood and peripheral blood of young adults displayed similar pattern of replicative senescence characterised by gradual decrease of proliferation capacity (assessed by CFSE assay) and reduction of CD28+ subpopulation of CD8+ cells. We were also interested whether CD8+CD28- were just progeny of CD28+ cells or if they were able to proliferate by themselves. After PHA stimulation of cells from adult donors at different ages, including centenarians, the transient up-regulation of CD28+ was observed. In CBMC and PBMC from young donors, the entire CD28+ subpopulation entered the cell cycle. In PBMC, from the majority of middle-aged subjects and all centenarians both CD28+ and CD28- were proliferating. All together we can conclude that in vitro CD8+CD28- are the progeny of both CD8+CD28+ and CD8+CD28- subpopulations. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.