Determination of benzimidazoles in pharmaceuticals and human serum by high-performance liquid chromatography

A liquid chromatographic method is described for the quantification of prednisolone, benzimidazoles, and preservatives using a C-18 analytical column as stationary phase. The mobile phase was 30:70 methanol:pH 2.5 phosphate buffer at a flow rate of 1.0mL min(-1) with absorbance detection at 235nm. The method was linear for concentrations ranged from 40-10,000ng mL(-1). Low values of coefficient of variance were obtained when samples were analyzed as replicates. Excellent recovery values were recorded in commercial products and fortified samples. International Conference of Harmonization protocols were employed to perform comprehensive method validation. The reported method has applications for pharmaceutical and serum samples.