New insights to primary photodynamic effects - Singlet oxygen kinetics in living cells

被引：93

作者：

Hackbarth, Steffen ^{[1
]}

Schlothauer, Jan ^{[1
]}

Preuss, Annegret ^{[1
]}

Roeder, Beate ^{[1
]}

机构：

[1] Humboldt Univ, Inst Phys, D-12489 Berlin, Germany

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 2010年 / 98卷 / 03期

关键词：

Singlet oxygen; Photodynamic therapy; Photosensitization; Quencher; Phototoxicity; Time correlated multi photon counting; TIME-RESOLVED DETECTION; LUMINESCENCE; LIFETIME; WATER; PHOTOSENSITIZATION; PHEOPHORBIDE; SUSPENSIONS; GENERATION; THERAPY; SKIN;

D O I：

10.1016/j.jphotobiol.2009.11.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The kinetics of chemical singlet oxygen quencher consumption inside living cells during low close illumination was revealed via time resolved singlet oxygen luminescence detection. Deviations of the measured data from the common theoretical model for O-1(2) kinetics forced the authors to consider a one-dimensional diffusion model for description of the kinetics of singlet oxygen generated by membrane localized photosensitizers. Our observations reconcile seemingly contradictory reports presenting different values for the efficiency of singlet oxygen interaction with cells. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：173 / 179

页数：7

共 34 条

[1]

[Anonymous], 2002, OXYGEN MOL MADE WORL

[2] Time-resolved investigations of singlet oxygen luminescence in water, in phosphatidylcholine, and in aqueous suspensions of phosphatidylcholine or HT29 cells [J].