Expression of soluble recombinant proteins in a cell-free system using a 96-well format

被引：34

作者：

Busso, D

Kim, R

Kim, SH

机构：

[1] Univ Calif Berkeley, Dept Chem, Calvin Lab 314, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Lawrence Berkeley Lab, Phys Biosci Div, Berkeley, CA 94720 USA

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2003年 / 55卷 / 03期

关键词：

cell-free; screening; expression; solubility; automation; recombinant proteins;

D O I：

10.1016/S0165-022X(03)00049-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

For structural and functional genomics programs, new high-throughput methods to obtain well-expressing and highly soluble proteins are essential. Here, we describe a rapid procedure to express recombinant proteins in an Escherichia coli cell-free system using a 96-well format. The identification of soluble proteins is performed by the Dot Blot procedure using an anti-His tag antibody. The applications and the automation of this method are described. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：233 / 240

页数：8

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