The poly(A) tail blocks RDR6 from converting self mRNAs into substrates for gene silencing

被引：53

作者：

Baeg, Kyungmin ^{[1
,2
]}

Iwakawa, Hiro-oki ^{[1
,2
]}

Tomari, Yukihide ^{[1
,2
]}

机构：

[1] Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 1130032, Japan

[2] Univ Tokyo, Grad Sch Frontier Sci, Dept Computat Biol & Med Sci, Bunkyo Ku, Tokyo 1130032, Japan

来源：

NATURE PLANTS | 2017年 / 3卷 / 04期

关键词：

SIRNA BIOGENESIS; INTERFERING RNAS; ARABIDOPSIS; TRANS; PLANTS; SUPPRESSION; SECONDARY;

D O I：

10.1038/nplants.2017.36

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

It remains unclear how post-transcriptional gene silencing (PTGS) in plants discriminates aberrant RNAs from canonical messenger RNAs (mRNAs). The key step of plant PTGS is the conversion of aberrant RNAs into double-stranded RNAs by RNA-DEPENDENT RNA POLYMERASE6 (RDR6). Here, we show that RDR6 itself selects aberrant poly(A)-less mRNAs over canonical polyadenylated mRNAs as templates at the initiation step of complementary strand synthesis. This mechanism can be viewed as an innate safeguard against 'self-attack' by PTGS.

引用

收藏

页数：4

相关论文

共 19 条

[1]

Allen E, 2005, CELL, V121, P207, DOI 10.1016/j.cell.2005.04.004

[2] The Slicer Activity of ARGONAUTE1 Is Required Specifically for the Phasing, Not Production, of Trans-Acting Short Interfering RNAs in Arabidopsis [J].

Arribas-Hernandez, Laura ;

Marchais, Antonin ;

Poulsen, Christian ;

Haase, Bettina ;

Hauptmann, Judith ;

Benes, Vladimir ;

Meister, Gunter ;

Brodersen, Peter .

PLANT CELL, 2016, 28 (07) :1563-1580

[3] A two-hit trigger for siRNA biogenesis in plants [J].

Axtell, Michael J. ;

Jan, Calvin ;

Rajagopalan, Ramya ;

Bartel, David P. .

CELL, 2006, 127 (03) :565-577

[4] The expanding world of small RNAs in plants [J].

Borges, Filipe ;

Martienssen, Robert A. .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2015, 16 (12) :727-741

[5] SKI2 mediates degradation of RISC 5′-cleavage fragments and prevents secondary siRNA production from miRNA targets in Arabidopsis [J].

Branscheid, Anja ;

Marchais, Antonin ;

Schott, Gregory ;

Lange, Heike ;

Gagliardi, Dominique ;

Andersen, Stig Uggerhoj ;

Voinnet, Olivier ;

Brodersen, Peter .

NUCLEIC ACIDS RESEARCH, 2015, 43 (22) :10975-10988

[6] An RNA-Dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus [J].

Dalmay, T ;

Hamilton, A ;

Rudd, S ;

Angell, S ;

Baulcombe, DC .

CELL, 2000, 101 (05) :543-553

[7] Phased, Secondary, Small Interfering RNAs in Posttranscriptional Regulatory Networks [J].

Fei, Qili ;

Xia, Rui ;

Meyers, Blake C. .

PLANT CELL, 2013, 25 (07) :2400-2415

[8] Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].

Fire, A ;

Xu, SQ ;

Montgomery, MK ;

Kostas, SA ;

Driver, SE ;

Mello, CC .

NATURE, 1998, 391 (6669) :806-811

[9] A link between rnRNA turnover and RNA interference in Arabidopsis [J].

Gazzani, S ;

Lawrenson, T ;

Woodward, C ;

Headon, D ;

Sablowski, R .

SCIENCE, 2004, 306 (5698) :1046-1048

[10] SGS3 and RDR6 interact and colocalize in cytoplasmic SGS3/RDR6-bodies [J].

Kumakura, Naoyoshi ;

Takeda, Atsushi ;

Fujioka, Yoichiro ;

Motose, Hiroyasu ;

Takano, Ryo ;

Watanabe, Yuichiro .

FEBS LETTERS, 2009, 583 (08) :1261-1266