A simple pH fluorescent probe based on new fluorophore indolizine for imaging of living cells

被引：77
作者：
Ge, Yanqing [1 ]
Liu, Aikun [1 ]
Dong, Jian [1 ]
Duan, Guiyun [1 ]
Cao, Xiaoqun [1 ]
Li, Fuyou [2 ,3 ,4 ]
机构：
[1] Taishan Med Univ, Sch Chem Engn, Tai An 271016, Shandong, Peoples R China
[2] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China
[3] Fudan Univ, State Key Lab Mol Engn Polymers, Shanghai 200433, Peoples R China
[4] Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2017年 / 247卷
基金：
中国国家自然科学基金;
关键词：
Indolizine; Bioimaging; ICT; Fluorescent probe; OPTICAL-PROPERTIES; RATIOMETRIC DETECTION; ACIDIC PH; TURN-ON; LYSOSOME; ICT; SENSOR; CHEMODOSIMETER; MITOCHONDRIA; CHEMOSENSOR;
D O I：
10.1016/j.snb.2017.02.157
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A simple pH fluorescent probe based on new fluorophore indolizine derivative has been synthesized and characterized. This probe responds to acidic pH (pKa = 3.85) with high quantum yield (phi = 0.52), short response time (within 1 min), high selectivity and sensitivity. The response mechanism of the fluorescent probes relies on ICT changes. The probe was successfully used to monitor intracellular H+ within RAW 264.7 cells. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码：46 / 52
页数：7
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