A sustainable strategy for effective regulation of aerobic granulation: Augmentation of the signaling molecule content by cultivating AHL-producing strains

被引:92
作者
Zhang, Bing [1 ,2 ]
Li, Wei [1 ]
Guo, Yuan [1 ]
Zhang, Zhiqiang [3 ]
Shi, Wenxin [4 ]
Cui, Fuyi [4 ]
Lens, Piet N. L. [5 ]
Tay, Joo Hwa [2 ]
机构
[1] Harbin Inst Technol, Sch Environm, Harbin 150090, Peoples R China
[2] Univ Calgary, Dept Civil Engn, Calgary, AB 72N 1N4, Canada
[3] Xian Univ Architecture & Technol, Sch Environm & Municipal Engn, Xian 710055, Peoples R China
[4] Chongqing Univ, Sch Environm & Ecol, Chongqing 400030, Peoples R China
[5] UNESCO IHE Inst Water Educ, Westvest 7, NL-2601 DA Delft, Netherlands
基金
中国国家自然科学基金;
关键词
Aerobic granular sludge (AGS); Quorum sensing (QS) regulation; N-acyl homoserine lactone (AHL)-Producing strains; Encapsulation; Protein; EXTRACELLULAR POLYMERIC SUBSTANCES; ACYL-HOMOSERINE LACTONES; SEQUENCING BATCH REACTOR; ACTIVATED-SLUDGE; MICROBIAL COMMUNITY; GRANULES; STABILITY; BACTERIA; FLOCS; NITRIFICATION;
D O I
10.1016/j.watres.2019.115193
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The positive roles of N-acyl homoserine lactone (AHL)-mediated quorum sensing (QS) in aerobic granular sludge (AGS) have been widely acknowledged. However, it is not feasible to manipulate granulation via direct addition of AHL chemicals or AHL-producing strains. Here, several strains with high AHL-producing capacity were successfully isolated from AGS. These QS strains were cultivated, mixed as a consortium, and then divided into two groups: AHLs supernatant and bacterial cells encapsulated in sodium alginate (CEBs). The potential of QS regulation, via doses of AHLs supernatant and CEBs, in accelerating granulation was evaluated. Results clearly indicated that short-term (days 21-70) addition of AHLs supernatant led to a rapid specific growth rate (0.08 d(-1)), compact structure without filamentous bacteria overgrowth, excellent settlement performance (SVl(10) 37.2 mL/g), and a high integrity coefficient (4.4%) of the granules. Sustainable release of AHLs (mainly C-6- and C-8-HSL) was induced by exogenous AHLs, possibly attributed to the enrichment of the genera Aeromonas and Pseudomonas. Further, tryptophan and aromatic protein substances were produced to maintain structural stability, suggesting that short-term QS regulation had long-term positive effects on the characteristics of AGS. By comparison, the addition of CEBs posed negligible or negative impact on the granulation, as evidenced by the rupture of smaller aggregates and poor characteristics of AGS. Overall, augmentation of the signaling content via addition of AHLs supernatant from QS strains is an economical and feasible regulation strategy to accelerate granulation and sustain long-term structural stability. (C) 2019 Elsevier Ltd. All rights reserved.
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页数:14
相关论文
共 53 条
[1]   Aerobic granular sludge: Recent advances [J].
Adav, Sunil S. ;
Lee, Duu-Jong ;
Show, Kuan-Yeow ;
Tay, Joo-Hwa .
BIOTECHNOLOGY ADVANCES, 2008, 26 (05) :411-423
[2]  
[Anonymous], 1995, STANDARD METHODS EXA
[3]   Enrichment of ANAMMOX bacteria from conventional activated sludge entrapped in poly(vinyl alcohol)/sodium alginate gel [J].
Bae, Hyokwan ;
Choi, Minkyu ;
Lee, Changsoo ;
Chung, Yun-Chul ;
Yoo, Young Je ;
Lee, Seockheon .
CHEMICAL ENGINEERING JOURNAL, 2015, 281 :531-540
[4]   N-Acyl-homoserine lactones and autoinducer-2-mediated quorum sensing during wastewater treatment [J].
Chen, Han ;
Li, Ang ;
Cui, Di ;
Wang, Qiao ;
Wu, Dan ;
Cui, Chongwei ;
Ma, Fang .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2018, 102 (03) :1119-1130
[5]   Physical properties and Extracellular Polymeric Substances pattern of aerobic granular sludge treating hypersaline wastewater [J].
Corsino, Santo Fabio ;
Capodici, Marco ;
Torregrossa, Michele ;
Viviani, Gaspare .
BIORESOURCE TECHNOLOGY, 2017, 229 :152-159
[6]   Investigation on extracellular polymeric substances, sludge flocs morphology, bound water release and dewatering performance of sewage sludge under pretreatment with modified phosphogypsum [J].
Dai, Quxiu ;
Ma, Liping ;
Ren, Nanqi ;
Ning, Ping ;
Guo, Zhiying ;
Xie, Longgui ;
Gao, Haijun .
WATER RESEARCH, 2018, 142 :337-346
[7]   A sustainable method for effective regulation of anaerobic granular sludge: Artificially increasing the concentration of signal molecules by cultivating a secreting strain [J].
Ding, Yangcheng ;
Feng, Huajun ;
Huang, Wenkun ;
Shen, Dongsheng ;
Wang, Meizhen .
BIORESOURCE TECHNOLOGY, 2015, 196 :273-278
[8]   Shifts in bacterial community composition and abundance of nitrifiers during aerobic granulation in two nitrifying sequencing batch reactors [J].
Fan, Xiao-Yan ;
Gao, Jing-Feng ;
Pan, Kai-Ling ;
Li, Ding-Chang ;
Zhang, Li-Fang ;
Wang, Shi-Jie .
BIORESOURCE TECHNOLOGY, 2018, 251 :99-107
[9]   Formation of aerobic granules and their PHB production at various substrate and ammonium concentrations [J].
Fang, Fang ;
Liu, Xian-Wei ;
Xu, Juan ;
Yu, Han-Qing ;
Li, Yong-Mei .
BIORESOURCE TECHNOLOGY, 2009, 100 (01) :59-63
[10]   Where are signal molecules likely to be located in anaerobic granular sludge? [J].
Feng, Huajun ;
Ding, Yangcheng ;
Wang, Meizhen ;
Zhou, Guanglan ;
Zheng, Xin ;
He, Hongzhen ;
Zhang, Xueqin ;
Shen, Dongsheng ;
Shentu, Jiali .
WATER RESEARCH, 2014, 50 :1-9