Monitoring cysteine level changes under LPS or H2O2 induced oxidative stress using a polymer-based ratiometric fluorescent probe

Cysteine (Cys) is a critical amino acid that involves in many physiological and pathological processes in the human body, and it plays an important role in maintaining redox homeostasis in living systems. The concentration of intracellular Cys is abnormal under oxidative stress thus leading to many diseases. Therefore, it is significant to develop an effective method for detection of Cys under oxidative stress. In this work, we propose a new polymer-based ratiometric fluorescent probe with good selectivity and sensitivity for detecting Cys. The bioimaging experiments results show that the novel probe has a rapid ratiometric response to Cys, which can be used to monitor Cys level changes during LPS or H2O2 induced oxidative stress in living cells and zebrafish. (C) 2021 Elsevier B.V. All rights reserved.