RNAi screening of human glycogene orthologs in the nematode Caenorhabditis elegans and the construction of the C-elegans glycogene database

被引：9

作者：

Akiyoshi, Sayaka ^{[1
]}

Nomura, Kazuko H. ^{[2
,3
]}

Dejima, Katsufumi ^{[2
,3
,4
]}

Murata, Daisuke ^{[1
,3
]}

Matsuda, Ayako ^{[1
]}

Kanaki, Nanako ^{[2
]}

Takaki, Tetsuro ^{[2
]}

Mihara, Hiroyuki ^{[2
]}

Nagaishi, Takayuki ^{[1
,2
]}

Furukawa, Shuhei ^{[2
]}

Ando, Keiko-Gengyo ^{[3
,4
]}

Yoshina, Sawako ^{[4
]}

Mitani, Shohei ^{[3
,4
]}

Togayachi, Akira ^{[5
]}

Suzuki, Yoshinori ^{[5
]}

Shikanai, Toshihide ^{[5
]}

Narimatsu, Hisashi ^{[5
]}

Nomura, Kazuya ^{[2
,3
]}

机构：

[1] Kyushu Univ, Fac Sci 33, Grad Sch Syst Life Sci, Fukuoka 8128581, Japan

[2] Kyushu Univ, Fac Sci 33, Dept Biol Sci, Fukuoka 8128581, Japan

[3] Japan Sci & Technol Agcy JST, CREST, Kawaguchi, Saitama 3320012, Japan

[4] Tokyo Womens Med Univ, Sch Med, Dept Physiol, Tokyo 1628666, Japan

[5] Natl Inst Adv Ind Sci & Technol, GTRC, Tsukuba, Ibaraki 3058568, Japan

来源：

GLYCOBIOLOGY | 2015年 / 25卷 / 01期

基金：

日本科学技术振兴机构;

关键词：

alg genes; germline cell division; glycogene; oligosaccharyltransferase; RNAi; LARVAL DEVELOPMENT; VULVAL MORPHOGENESIS; ESSENTIAL ROLES; GENES; EXPRESSION; FAMILY; CLASSIFICATION; DISTINCT; GLYCOSYLTRANSFERASES; IDENTIFICATION;

D O I：

10.1093/glycob/cwu080

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, we selected 181 nematode glycogenes that are orthologous to human glycogenes and examined their RNAi phenotypes. The results are deposited in the Caenorhabditis elegans Glycogene Database (CGGDB) at AIST, Tsukuba, Japan. The most prominent RNAi phenotypes observed are disruptions of cell cycle progression in germline mitosis/meiosis and in early embryonic cell mitosis. Along with the previously reported roles of chondroitin proteoglycans, glycosphingolipids and GPI-anchored proteins in cell cycle progression, we show for the first time that the inhibition of the functions of N-glycan synthesis genes (cytoplasmic alg genes) resulted in abnormal germline formation, ER stress and small body size phenotypes. The results provide additional information on the roles of glycoconjugates in the cell cycle progression mechanisms of germline and embryonic cells.

引用

页码：8 / +

页数：13

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