Is monosomy 5 an uncommon aberration?: Fluorescence in situ hybridization reveals translocations and deletions in myelodysplastic syndromes or acute myelocytic leukemia

被引:17
作者
Bram, S
Swolin, B [1 ]
Rödjer, S
Stockelberg, D
Ögärd, I
Bäck, H
机构
[1] Sahlgrens Univ Hosp, Dept Clin Chem & Transfus Med, S-41345 Gothenburg, Sweden
[2] Sahlgrens Univ Hosp, Dept Med, Sect Hematol, S-41345 Gothenburg, Sweden
[3] Boras Hosp, Dept Med, Boras, Sweden
关键词
D O I
10.1016/S0165-4608(02)00836-1
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Acquired loss of material from chromosome 5 in bone marrow cells is common in myelodysplastic syndromes (MDS) and acute myelocytic leukemia (AML). In this study, we have applied fluorescence in situ hybridization (FISH) analyses with probes for the three regions 5p15.2, 5q31, 5q33similar toq34, and whole chromosome 5 painting probes (WCP 5) to investigate what further information could be gained regarding the cytogenetic abnormalities of chromosome 5 in 35 patients with MDS or AML. With FISH, a del(5q) was found in all patients except for two. Translocations of material from chromosome 5 were found in 10 patients. Among 16 patients with clones of monosomy 5 seen by cytogenetics, 14 had deletions or translocations. Different breakpoints on chromosome 5 were observed. In conclusion, the extended FISH analyses yielded additional information about chromosome 5 abnormalities in 60% of the patients. Of interest is the finding of a high proportion of translocations and that monosomy 5 occurs less often than is generally believed. (C) 2003 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:107 / 114
页数:8
相关论文
共 31 条
[1]  
BENNETT JM, 1982, BRIT J HAEMATOL, V51, P189, DOI 10.1111/j.1365-2141.1982.tb08475.x
[2]   PROPOSALS FOR CLASSIFICATION OF ACUTE LEUKEMIAS [J].
BENNETT, JM ;
CATOVSKY, D ;
DANIEL, MT ;
FLANDRIN, G ;
GALTON, DAG ;
GRALNICK, HR ;
SULTAN, C .
BRITISH JOURNAL OF HAEMATOLOGY, 1976, 33 (04) :451-&
[3]   PROPOSED REVISED CRITERIA FOR THE CLASSIFICATION OF ACUTE MYELOID-LEUKEMIA - A REPORT OF THE FRENCH-AMERICAN-BRITISH COOPERATIVE GROUP [J].
BENNETT, JM ;
CATOVSKY, D ;
DANIEL, MT ;
FLANDRIN, G ;
GALTON, DAG ;
GRALNICK, HR ;
SULTAN, C .
ANNALS OF INTERNAL MEDICINE, 1985, 103 (04) :620-625
[4]   KARYOTYPE IN MYELODYSPLASTIC SYNDROMES - RELATIONS TO MORPHOLOGY, CLINICAL EVOLUTION, AND SURVIVAL [J].
BERNASCONI, P ;
ALESSANDRINO, EP ;
BONI, M ;
BONFICHI, M ;
MORRA, E ;
LAZZARINO, M ;
CAMPAGNOLI, C ;
ASTORI, C .
AMERICAN JOURNAL OF HEMATOLOGY, 1994, 46 (04) :270-277
[5]   CHROMOSOMAL DELETIONS IN MYELODYSPLASIA [J].
BOULTWOOD, J ;
FIDLER, C .
LEUKEMIA & LYMPHOMA, 1995, 17 (1-2) :71-78
[6]  
BOULTWOOD J, 1994, BLOOD, V84, P3253
[7]   Fluorescence in situ hybridization confirmation of 5q deletions in patients with hematological malignancies [J].
Brezinová, J ;
Zemanová, Z ;
Cermák, J ;
Michalová, K .
CANCER GENETICS AND CYTOGENETICS, 2000, 117 (01) :45-49
[8]   Translocations and deletions of 5q13.1 in myelodysplasia and acute myelogenous leukemia: Evidence for a novel critical locus [J].
Fairman, J ;
Wang, RY ;
Liang, H ;
Zhao, L ;
Saltman, D ;
Liang, JC ;
Nagarajan, L .
BLOOD, 1996, 88 (06) :2259-2266
[9]   Validation of the WHO proposals for a new classification of primary myelodysplastic syndromes: a retrospective analysis of 1600 patients [J].
Germing, U ;
Gattermann, N ;
Strupp, C ;
Aivado, M ;
Aul, C .
LEUKEMIA RESEARCH, 2000, 24 (12) :983-992
[10]   International scoring system for evaluating prognosis in myelodysplastic syndromes [J].
Greenberg, P ;
Cox, C ;
LeBeau, MM ;
Fenaux, P ;
Morel, P ;
Sanz, G ;
Sanz, M ;
Vallespi, T ;
Hamblin, T ;
Oscier, D ;
Ohyashiki, K ;
Toyama, K ;
Aul, C ;
Mufti, G ;
Bennett, J .
BLOOD, 1997, 89 (06) :2079-2088