Electrochemically induced surface-enhanced infrared difference absorption (SEIDA) spectroscopy of a protein monolayer

被引：69
作者：
Ataka, K [1 ]
Heberle, J [1 ]
机构：
[1] Forschungszentrum Julich, D-52425 Julich, Germany
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 17期
关键词：
D O I：
10.1021/ja0346532
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Functional infrared difference spectra of a protein monolayer are recorded by exploiting the surface enhancement exerted by a modified gold surface. The noble metal is used as the working electrode to induce redox-linked structural changes in the single layer of cytochrome c. The observed differences in the relative band intensities as compared to the difference spectrum of cytochrome c in solution are attributed to orientational effects of the adsorbed protein. Copyright © 2003 American Chemical Society.
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页码：4986 / 4987
页数：2
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