Yellow and Orange Fluorescent Proteins with Tryptophan-based Chromophores

被引：7

作者：

Bozhanova, Nina G. ^{[1
]}

Baranov, Mikhail S. ^{[1
,2
]}

Sarkisyan, Karen S. ^{[1
]}

Gritcenko, Roman ^{[3
]}

Mineev, Konstantin S. ^{[1
,4
]}

Golodukhina, Svetlana V. ^{[1
]}

Baleeva, Nadezhda S. ^{[1
,2
]}

Lukyanov, Konstantin A. ^{[1
]}

Mishin, Alexander S. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Bioorgan Chem, Miklukho Maklaya 16-10, Moscow 117997, Russia

[2] Pirogov Russian Natl Res Med Univ, Ostrovitianov 1, Moscow 117997, Russia

[3] Lund Univ, Dept Chem, Ctr Anal & Synth, S-22100 Lund, Sweden

[4] Moscow Inst Phys & Technol, Inst Sky Per 9, Dolgoprudnyi 141701, Russia

来源：

ACS CHEMICAL BIOLOGY | 2017年 / 12卷 / 07期

基金：

俄罗斯基础研究基金会;

关键词：

ANEMONIA-SULCATA; MONOMERIC RED; MATURATION; MECHANISM; MKATE;

D O I：

10.1021/acschembio.7b00337

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rapid development of new microscopy technicques exposed the heed for genetically encoded fluorescent tags with special properties. Recent works demonstrated the potential of fluorescent proteins with tryptophan-based chromophores We applied rational design and random mutagenesis to the monomeric red fluorescent protein FusionRed and found two groups of mutants carrying a tryptophan-teased chromophore: with yellow (535 nm) or orange (565 nm) emission. On the basis of the properties of proteins, a model synthetic chromophore, and a computational modeling, we concluded that the presence of a ketone-containing chromophore in different isomeric forms can explain the observed yellow and orange phenotypes.

引用

页码：1867 / 1873

页数：7

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