Mass spectrometric characterization of a two-glycosyltransferase tandem reaction for assembly of tetrasaccharide repeating unit of Escherichia coli O77 O-antigen

被引：3
作者：
Zhou, Dawei [1 ]
Chen, Chao [1 ]
Xu, Lingling [1 ]
Utkina, Natalia [2 ]
Danilov, Leonid [2 ]
Torgov, Vladimir [2 ]
Veselovsky, Vladimir [2 ]
Liu, Bin [1 ]
Feng, Lu [1 ]
机构：
[1] Nankai Univ, TEDA Inst Biol Sci & Biotechnol, TEDA, 23 Hongda St, Tianjin 300457, Peoples R China
[2] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Leninsky Prosp 47, Moscow 119991, Russia
来源：
CARBOHYDRATE RESEARCH | 2016年 / 424卷
基金：
中国国家自然科学基金; 对外科技合作项目（国际科技项目）;
关键词：
E. coli O77; O-antigen repeating unit; Glycosyltransferases; WbaD; WbaC; Electrospray ionization multistage tandem mass spectrometry; BIOCHEMICAL-CHARACTERIZATION; BIOSYNTHESIS; IDENTIFICATION; OLIGOSACCHARIDES; ARABINOFURANOSYLTRANSFERASE; LIPOPOLYSACCHARIDE; POLYSACCHARIDE; ASSOCIATIONS; SEROGROUPS; TOXINS;
D O I：
10.1016/j.carres.2016.02.007
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The wbaD gene and wbaC gene from Escherichia coli O77 O-antigen gene cluster encoding mannosyltransferases were functionally characterized in vitro. A synthetic acceptor P-1-(11-phenoxyundecyl)-P-2-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl) diphosphate (GlcNAc-PP-PhU) was used as an acceptor and GDP-Man as a donor substrate; the activities of WbaD and WbaC were confirmed by detailed structural characterization of their lipooligosacharide enzyme products using high-sensitivity negative-ion electrospray ionization (ESI) collision-induced dissociation tandem mass spectrometry (CID) MS-MS. The extensive fragmentation unequivocally demonstrated that the Man(1-3)-GlcNAc linkage in WbaD catalyzed reaction product and two Man(1-2)-Man linkages in tandem WbaD/WbaC catalyzed reaction product are present, respectively. This study provided valuable information for the understanding of diversified glycosyltransferase (GT) functions and the two GTs characterized can serve as additional enzyme sources for possible pharmaceutical related applications. (C) 2016 Elsevier Ltd. All rights reserved.
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页码：24 / 29
页数：6
相关论文
共 24 条
  • [11] ANALYSIS OF CARBOHYDRATES AND GLYCOCONJUGATES BY MATRIX-ASSISTED LASER DESORPTION/IONIZATION MASS SPECTROMETRY: AN UPDATE FOR 2003-2004
    Harvey, David J.
    [J]. MASS SPECTROMETRY REVIEWS, 2009, 28 (02) : 273 - 361
  • [12] Identification of a glycosylphosphatidylinositol anchor-modifying β1-3 N-acetylglucosaminyl transferase in Trypanosoma brucei
    Izquierdo, Luis
    Nakanishi, Masayuki
    Mehlert, Angela
    Machray, Greg
    Barton, Geoffrey J.
    Ferguson, Michael A. J.
    [J]. MOLECULAR MICROBIOLOGY, 2009, 71 (02) : 478 - 491
  • [13] Biosynthesis of mycobacterial lipoarabinomannan: Role of a branching mannosyltransferase
    Kaur, Devinder
    Berg, Stefan
    Dinadayala, Premkumar
    Gicquel, Brigitte
    Chatterjee, Delphi
    McNeil, Michael R.
    Vissa, Varalakshmi D.
    Crick, Dean C.
    Jackson, Mary
    Brennan, Patrick J.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (37) : 13664 - 13669
  • [14] Role of structural variations of polysaccharide antigens in the pathogenicity of Gram-negative bacteria
    Lukacova, M.
    Barak, I.
    Kazar, J.
    [J]. CLINICAL MICROBIOLOGY AND INFECTION, 2008, 14 (03) : 200 - 206
  • [15] Structural characterization of oligosaccharides using MALDI-TOF/TOF tandem mass spectrometry
    Mechref, Y
    Novotny, MV
    Krishnan, C
    [J]. ANALYTICAL CHEMISTRY, 2003, 75 (18) : 4895 - 4903
  • [16] Prevalence and characterization of Shiga toxin-producing Escherichia coli isolated from cattle, food, and children during a one-year prospective study in France
    Pradel, N
    Livrelli, V
    De Champs, C
    Palcoux, JB
    Reynaud, A
    Scheutz, F
    Sirot, J
    Joly, B
    Forestier, C
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (03) : 1023 - 1031
  • [17] ROLE OF O-ANTIGEN VARIATION IN THE IMMUNE-RESPONSE
    REEVES, P
    [J]. TRENDS IN MICROBIOLOGY, 1995, 3 (10) : 381 - 386
  • [18] Sambrook J., 1989, MOL CLONING LABORATO, P201
  • [19] Biosynthesis of O-antigens: genes and pathways involved in nucleotide sugar precursor synthesis and O-antigen assembly
    Samuel, G
    Reeves, P
    [J]. CARBOHYDRATE RESEARCH, 2003, 338 (23) : 2503 - 2519
  • [20] A group of Escherichia coli and Salmonella enterica O antigens sharing a common backbone structure
    Wang, Wei
    Perepelov, Andrei V.
    Feng, Lu
    Shevelev, Sergei D.
    Wang, Quan
    Senchenkova, Sof'ya N.
    Han, Weiqing
    Li, Yayue
    Shashkov, Alexander S.
    Knirel, Yuriy A.
    Reeves, Peter R.
    Wang, Lei
    [J]. MICROBIOLOGY-SGM, 2007, 153 : 2159 - 2167
123