Study of oligonucleotide fixation on bilayer lipid membranes by patch-clamp pipette support

被引：0
|
作者：
Liu, Nan [1 ]
Gao, Zhixian [1 ]
Zhou, Huanying [1 ]
He, Yonghong [1 ]
Yue, Mingxiang [1 ]
机构：
[1] Acad Mil Med Sci, Inst Hyg & Environm Med, Tianjin 300050, Peoples R China
来源：
RARE METAL MATERIALS AND ENGINEERING | 2006年 / 35卷
关键词：
bilayer lipid membranes; patch-clamp pipette; oligonucleotide;
D O I：
暂无
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
One kind of novel BLMs was fabricated by patch-clamp pipette technology characterized in considerably sensitive to changes of electrochemical parameters. Detective currents and voltage presented linear relationship when BLMs was formed and it could be confirmed by Gramicidin method. Ion current was increased by dihexyl(C-12) modified ssDNA fixed on the BLMs and also indicated linear relationship to ssDNA's concentration due to the interaction Of C-12-ssDNA and BLMs. Further more, the regression equations were different from BLMs fixed with ssDNA probe and a blank control BLM in the same experimental conditions. The ssDNA probe was successfully fixed on patch-clamp pipette supported-BLMs. Based on our studies, a biosensor with reactive element of patch-clamp pipette-supported BLMs has been established.
引用
收藏
页码：324 / 326
页数：3
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