Microsatellite DNA markers for the pea aphid Acyrthosiphon pisum

被引：26
|
作者：
Caillaud, MC [1 ]
Mondor-Genson, G
Levine-Wilkinson, S
Mieuzet, L
Frantz, A
Simon, JC
Coeur d'Acier, A
机构：
[1] Ithaca Coll, Dept Biol, Ctr Nat Sci 165, Ithaca, NY 14853 USA
[2] CBGP, F-34988 Montferrier Sur Lez, France
[3] INRA, UMR, ENSAR Bio3P, F-35653 Le Rheu, France
来源：
MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 03期
关键词：
cross priming; dinucleotide repeats; enriched library; microsatellite; pea aphid;
D O I：
10.1111/j.1471-8286.2004.00676.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Microsatellite loci were isolated from enriched partial genomic libraries of Acyrthosiphon loti and Acyrthosiphon pisum. Twenty of those loci were characterized in A. pisum. Fifteen of those loci were polymorphic. Genetic diversity varied across loci, allele repeat number ranging from two to 15, and observed heterozygosity from 0.1 and 0.96. An additional eight microsatellite loci originally isolated from other aphids but cross-priming with A. pisum showed polymorphism as well. Allele size ranged from three to 9 and observed heterozygosity from 0.43 to 0.84. Overall, we present 23 microsatellite loci that can be used to reveal polymorphism in pea aphids.
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页码：446 / 448
页数：3
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