The bZIP transcription factor MdHY5 regulates anthocyanin accumulation and nitrate assimilation in apple

被引：313

作者：

An, Jian-Ping ^{[1
]}

Qu, Feng-Jia ^{[1
]}

Yao, Ji-Fang ^{[1
]}

Wang, Xiao-Na ^{[2
]}

You, Chun-Xiang ^{[1
]}

Wang, Xiao-Fei ^{[1
]}

Hao, Yu-Jin ^{[1
]}

机构：

[1] Shandong Agr Univ, Coll Hort Sci & Engn, MOA Key Lab Hort Crop Biol & Germplasm Innovat, Natl Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China

[2] Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China

来源：

HORTICULTURE RESEARCH | 2017年 / 4卷

关键词：

HY5 PHYSICALLY INTERACT; RED COLORATION; UV-B; POSTTRANSCRIPTIONAL REGULATION; BIOSYNTHETIC GENES; SALICYLIC-ACID; UPTAKE SYSTEM; ARABIDOPSIS; LIGHT; EXPRESSION;

D O I：

10.1038/hortres.2017.23

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The basic leucine zipper (bZIP) transcription factor HY5 plays a multifaceted role in plant growth and development. Here the apple MdHY5 gene was cloned based on its homology with Arabidopsis HY5. Expression analysis demonstrated that MdHY5 transcription was induced by light and abscisic acid treatments. Electrophoretic mobility shift assays and transient expression assays subsequently showed that MdHY5 positively regulated both its own transcription and that of MdMYB10 by binding to E-box and G-box motifs, respectively. Furthermore, we obtained transgenic apple calli that overexpressed the MdHY5 gene, and apple calli coloration assays showed that MdHY5 promoted anthocyanin accumulation by regulating expression of the MdMYB10 gene and downstream anthocyanin biosynthesis genes. In addition, the transcript levels of a series of nitrate reductase genes and nitrate uptake genes in both wild-type and transgenic apple calli were detected. In association with increased nitrate reductase activities and nitrate contents, the results indicated that MdHY5 might be an important regulator in nutrient assimilation. Taken together, these results indicate that MdHY5 plays a vital role in anthocyanin accumulation and nitrate assimilation in apple.

引用

页数：9

共 60 条

[1] Arabidopsis CAM7 and HY5 Physically Interact and Directly Bind to the HY5 Promoter to Regulate Its Expression and Thereby Promote Photomorphogenesis [J].