Sequence and expression of four coral G protein-coupled receptors distinct from all classifiable members of the rhodopsin family

被引:21
作者
Anctil, Michel
Hayward, David C.
Miller, David J.
Ball, Eldon E.
机构
[1] Univ Montreal, Dept Sci Biol, Montreal, PQ H3C 3J7, Canada
[2] Australian Natl Univ, Res Sch Biol Sci, Ctr Mol Genet Dev, Canberra, ACT 2601, Australia
[3] James Cook Univ N Queensland, Comparat Genom Ctr, Townsville, Qld 4811, Australia
基金
加拿大自然科学与工程研究理事会; 澳大利亚研究理事会;
关键词
GPCR; Cnidaria; Anthozoa; Acropora; orphan receptor;
D O I
10.1016/j.gene.2006.10.025
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
A measure of the functional importance of G protein-coupled receptors (GPCRs) as signalling molecules is that over seven hundred have been cloned and identified in the human genome alone. Yet few have been characterized in the lower metazoan phyla, especially in the phylum Cnidaria which is well positioned phylogenetically for tracing the early evolution of GPCRs owing to their possession of the first-evolved nervous systems. We report here the cloning and characterization of four novel rhodopsin-like GPCR cDNAs from the staghorn coral Acropora millepora that share significant similarity with each other but not with the majority of other members of the rhodopsin a subfamily. The deduced proteins lack many of the conserved residues and motifs that form the signature of the different groups of alpha rhodopsin receptors. Maximum likelihood phylogenetic analysis likewise implies that the coral receptors do not have a simple or close relationship with any of the major groups within the alpha rhodopsin subfamily. In situ hybridization revealed transcripts in endodermal cells of planula larvae of all ages and in post-settlement polyps. These GPCRs appear to belong to a alpha rhodopsin-like group unique to corals. Comparisons with other cnidarian GPCRs suggest also that GPCRs diverged early in metazoan evolution. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:14 / 21
页数:8
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