Confocal light absorption and scattering spectroscopic microscopy

被引：49

作者：

Fang, Hui ^{[1
]}

Qiu, Le

Vitkin, Edward

Zaman, Munir M.

Andersson, Charlotte

Salahuddin, Saira

Kimerer, Lauren M.

Cipolloni, Patsy B.

Modell, Mark D.

Turner, Bradley S.

Keates, Sarah E.

Bigio, Irving

Itzkan, Irving

Freedman, Steven D.

Bansil, Rama

Hanlon, Eugene B.

Perelman, Lev T.

机构：

[1] Harvard Univ, Sch Med, Biomed Imaging & Spect Lab, Beth Israel Deaconess Med Ctr,Dept Med, Boston, MA 02215 USA

[2] Harvard Univ, Sch Med, Biomed Imaging & Spect Lab, Beth Israel Deaconess Med Ctr,Dept Obgyn & Reprod, Boston, MA 02215 USA

[3] Ctr Geriatr Res Educ & Clin, Bedford, MA 01730 USA

[4] Dept Vet Affairs, Med Res Serv, Bedford, MA 01730 USA

[5] Boston Univ, Ctr Polymer Studies, Boston, MA 02215 USA

[6] Boston Univ, Dept Phys, Boston, MA 02215 USA

[7] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA

[8] Boston Univ, Dept Elect & Comp Engn, Boston, MA 02215 USA

来源：

APPLIED OPTICS | 2007年 / 46卷 / 10期

关键词：

D O I：

10.1364/AO.46.001760

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We have developed a novel optical method for observing submicrometer intracellular structures in living cells, which is called confocal light absorption and scattering spectroscopic (CLASS) microscopy. It combines confocal microscopy, a well-established high-resolution microscopic technique, with light-scattering spectroscopy. CLASS microscopy requires no exogenous labels and is capable of imaging and continuously monitoring individual viable cells, enabling the observation of cell and organelle functioning at scales of the order of 100 nm. (c) 2007 Optical Society of America.

引用

页码：1760 / 1769

页数：10

共 36 条

[21] Development and use of fluorescent protein markers in living cells [J].

Lippincott-Schwartz, J ;

Patterson, GH .

SCIENCE, 2003, 300 (5616) :87-91

[22] Elastic backscattering spectroscopic microscopy [J].

Liu, Y ;

Li, X ;

Kim, YL ;

Backman, V .

OPTICS LETTERS, 2005, 30 (18) :2445-2447

[23] SIZE AND REFRACTIVE-INDEX DETERMINATION OF SINGLE POLYSTYRENE SPHERES [J].

MARX, E ;

MULHOLLAND, GW .

JOURNAL OF RESEARCH OF THE NATIONAL BUREAU OF STANDARDS, 1983, 88 (05) :321-338

[24]

Mishchenko M., 2000, LIGHT SCATTERING NON

[25]

MONIKA G, 2001, AM J PHYSIOL-GASTR L, V281, P823

[26] Observation of periodic fine structure in reflectance from biological tissue: A new technique for measuring nuclear size distribution [J].

Perelman, LT ;

Backman, V ;

Wallace, M ;

Zonios, G ;

Manoharan, R ;

Nusrat, A ;

Shields, S ;

Seiler, M ;

Lima, C ;

Hamano, T ;

Itzkan, I ;

Van Dam, J ;

Crawford, JM ;

Feld, MS .

PHYSICAL REVIEW LETTERS, 1998, 80 (03) :627-630

[27] Optical spectroscopy noninvasively monitors response of organelles to cellular stress [J].

Schuele, G ;

Vitkin, E ;

Huie, P ;

O'Connell-Rodwell, C ;

Palanker, D ;

Perelman, LT .

JOURNAL OF BIOMEDICAL OPTICS, 2005, 10 (05)

[28] A guide to choosing fluorescent proteins [J].

Shaner, NC ;

Steinbach, PA ;

Tsien, RY .

NATURE METHODS, 2005, 2 (12) :905-909

[29] Endoscopic detection of dysplasia in patients with Barrett's esophagus using light-scattering spectroscopy [J].

Wallace, MB ;

Perelman, LT ;

Backman, V ;

Crawford, JM ;

Fitzmaurice, M ;

Seiler, M ;

Badizadegan, K ;

Shields, SJ ;

Itzkan, I ;

Dasari, RR ;

Van Dam, J ;

Feld, MS .

GASTROENTEROLOGY, 2000, 119 (03) :677-682

[30] Imaging of spheres with the confocal scanning optical microscope [J].

Weise, W ;

Zinin, P ;

Wilson, T ;

Briggs, GAD ;

Boseck, S .

OPTICS LETTERS, 1996, 21 (22) :1800-1802

← 1 2 3 4 →