Development of collagen/nanohydroxyapatite scaffolds containing plant extract intended for bone regeneration

被引：23
作者：
Garcia, Claudio Fernandes [1 ]
Marangon, Crisiane Aparecida [2 ]
Massimino, Livia Contini [2 ]
Klingbeil, Maria Fatima Guarizo [3 ]
Martins, Virginia Conceicao Amaro [1 ]
Plepis, Ana Maria de Guzzi [1 ,2 ]
机构：
[1] Univ Sao Paulo, Sao Carlos Inst Chem IQSC, BR-13560970 Sao Carlos, Brazil
[2] Univ Sao Paulo, Interunits Grad Program Bioengn EESC FMRP IQSC, BR-13560970 Sao Carlos, Brazil
[3] Nucl & Energy Res Inst Ipen, BR-05508000 Sao Paulo, Brazil
来源：
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2021年 / 123卷 / 123期
关键词：
Collagen; Nanohydroxyapatite; Flavonoids; Bone regeneration; CROSS-LINKING; GRAPE SEED; HYDROXYAPATITE; ANTIOXIDANT; PEEL; PROANTHOCYANIDINS; BIOMATERIALS; CHITOSAN; POWDER;
D O I：
10.1016/j.msec.2021.111955
中图分类号：
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号：
0805 ; 080501 ; 080502 ;
摘要：
In this study scaffolds of nanohydroxyapatite (nHA) and anionic collagen (C) combined with plant extracts intended for bone tissue repair were developed. Grape seed (P), pomegranate peel (R) and jabuticaba peel (J) extracts were used as collagen crosslinker agents in order to improve the materials properties. All crude extracts were effective against Staphylococcus aureus, but only for CR scaffold inhibition zone was noticed. The extracts acted as crosslinking agents, increasing enzymatic resistance and thermal stability of collagen. The extracts showed cytotoxicity at the concentrations tested, while nHA increased cell viability. The scaffolds presented porosity and pore size appropriate for bone growth. CR, CnHAP, CnHAR and CnHAJ increased the cell viability after 24 h. The combination of collagen, nHA and plant extracts offers a promising strategy to design novel biomaterials for bone tissue regeneration.
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页数：12
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