Antenatal taurine improves neuronal regeneration in fetal rats with intrauterine growth restriction by inhibiting the Rho-ROCK signal pathway

被引：28
作者：
Liu, Jing [1 ,2 ]
Wang, Hua-Wei [1 ,2 ,3 ]
Liu, Fang [1 ,2 ,4 ]
Wang, Xiao-Feng [1 ,2 ]
机构：
[1] Beijing Mil Gen Hosp, Bayi Childrens Hosp, Dept Neonatol, Beijing 100700, Peoples R China
[2] Beijing Mil Gen Hosp, Bayi Childrens Hosp, NICU, Beijing 100700, Peoples R China
[3] Anhui Med Univ, Grad Sch, Hefei 230033, Peoples R China
[4] Southern Med Univ, Grad Sch, Guangzhou 510515, Guangdong, Peoples R China
来源：
METABOLIC BRAIN DISEASE | 2015年 / 30卷 / 01期
关键词：
Intrauterine growth restriction; Taurine; Rho-ROCK signal pathway; CENTRAL-NERVOUS-SYSTEM; LOW-PROTEIN DIET; RHO/ROCK PATHWAY; IN-VITRO; BRAIN; SUPPLEMENTATION; ACTIVATION; APOPTOSIS; INJURY; MODEL;
D O I：
10.1007/s11011-014-9572-x
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The Rho-ROCK signal pathway is an important mediator of inhibitory signals that blocks central nervous cell regeneration. Here, we investigated whether antenatal taurine improved neuronal regeneration in fetal rats with intrauterine growth restriction (IUGR) by inhibiting this pathway. Thirty pregnant rats were randomly divided into three groups: control, IUGR, and IUGR + antenatal taurine supplementation (taurine group). The mRNA levels of Ras homolog gene A (Rho A), Rho-associated coiled-coil forming protein kinase 2 (ROCK2), and proliferating cell nuclear antigen (PCNA) were detected using real-time quantitative PCR. RhoA, ROCK2 and PCNA-positive cells were counted using immunohistochemistry. Antenatal taurine supplementation decreased RhoA and Rock2 mRNA expression, increased PCNA mRNA expression, and significantly decreased RhoA, ROCK2-positive and increased PCNA-positive cell counts in IUGR fetal rat brain tissues (p < 0.05). Thus, antenatal taurine supplementation inhibited the expression of key Rho-ROCK signal molecules and improved IUGR fetal brain development.
引用
收藏
页码：67 / 73
页数：7
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