New derivative of β-cyclodextrin as chiral selectors for the capillary electrophoretic separation of chiral drugs

The uncharged beta-cyclodextrin (beta-CD) derivative mono-3-O-phenylcarbamoyl-beta-CD was successfully used as chiral selector in capillary zone electrophoresis. Several chiral drugs such as propafenone and verapamil enantiomers belonging to different classes of compounds of pharmaceutical interest were resolved. The chiral resolution (Rs) was strongly influenced by the concentrations of the cyclodextrin derivative, the background electrolyte, and the pH of the background electrolyte. The influence of organic modifier was also studied. Under the conditions of 40 mmol/l berate buffer at pH 9.18 containing 2.5 mmol/l mono-3-phenylcarbamoyl-beta-CD, baseline separation (Rs=3.88 for verapamil, Rs=3.32 for propafenone) of the enantiomers can be achieved. (C) 2000 Elsevier Science B.V. All rights reserved.