Frzb-1 is a secreted antagonist of Wnt signaling expressed in the Spemann organizer

被引：626

作者：

Leyns, L ^{[1
]}

Bouwmeester, T ^{[1
]}

Kim, SH ^{[1
]}

Piccolo, S ^{[1
]}

DeRobertis, EM ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,DEPT BIOL CHEM,LOS ANGELES,CA 90095

来源：

CELL | 1997年 / 88卷 / 06期

关键词：

D O I：

10.1016/S0092-8674(00)81921-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Frzb-1 is a secreted protein containing a domain similar to the putative Wnt-binding region of the frizzled family of transmembrane receptors. Frzb-1 is widely expressed in adult mammalian tissues. In the Xenopus gastrula, it is expressed and regulated as a typical Spemann organizer component. Injection of frzb-1 mRNA blocks expression of XMyoD mRNA and leads to embryos with enlarged heads and shortened trunks. Frzb-1 antagonizes the effects of Xwnt-8 ectopic expression in a non-cell-autonomous manner. Cultured cells transfected with a membrane-tethered form of Wnt-1 bind epitope-tagged Frzb-1 in the 10(-10) M range. The results strengthen the view that the Spemann organizer is a source of secreted inhibitory factors.

引用

页码：747 / 756

页数：10

共 46 条

[1] A new member of the frizzled family from Drosophila functions as a Wingless receptor [J].

Bhanot, P ;

Brink, M ;

Samos, CH ;

Hsieh, JC ;

Wang, YS ;

Macke, JP ;

Andrew, D ;

Nathans, J ;

Nusse, R .

NATURE, 1996, 382 (6588) :225-230

[2] Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer [J].

Bouwmeester, T ;

Kim, SH ;

Sasai, Y ;

Lu, B ;

DeRobertis, EM .

NATURE, 1996, 382 (6592) :595-601

[3] BIOCHEMICAL-ANALYSIS OF MURINE WNT PROTEINS REVEALS BOTH SHARED AND DISTINCT PROPERTIES [J].

BURRUS, LW ;

MCMAHON, AP .

EXPERIMENTAL CELL RESEARCH, 1995, 220 (02) :363-373

[4]

CHAKRABARTI A, 1992, DEVELOPMENT, V115, P355

[5]

CHAN SDH, 1992, J BIOL CHEM, V267, P25202

[6]

CHRISTIAN JL, 1991, DEVELOPMENT, V111, P1045

[7] INTERACTIONS BETWEEN XWNT-8 AND SPEMANN ORGANIZER SIGNALING PATHWAYS GENERATE DORSOVENTRAL PATTERN IN THE EMBRYONIC MESODERM OF XENOPUS [J].

CHRISTIAN, JL ;

MOON, RT .

GENES & DEVELOPMENT, 1993, 7 (01) :13-28

[8]

CUI YZ, 1995, DEVELOPMENT, V121, P2177

[9] A common plan for dorsoventral patterning in Bilateria [J].

DeRobertis, EM ;

Sasai, Y .

NATURE, 1996, 380 (6569) :37-40

[10]

DU SJ, 1995, MOL CELL BIOL, V15, P2625

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