Mammalian cell transfection: the present and the future

被引：446

作者：

Kim, Tae Kyung ^{[2
]}

Eberwine, James H. ^{[1
,2
]}

机构：

[1] Univ Penn, Penn Genome Frontiers Inst, Med Sch Pharmacol, Philadelphia, PA 19104 USA

[2] Univ Penn, Dept Pharmacol, Med Sch Pharmacol, Philadelphia, PA 19104 USA

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2010年 / 397卷 / 08期

关键词：

Transfection; Nucleic acid; Gene; Single cell; GENE-TRANSFER; IN-VIVO; MEDIATED TRANSFECTION; SYMPATHETIC NEURONS; DELIVERY; THERAPY; RNA; EXPRESSION; PROSPECTS; BRAIN;

D O I：

10.1007/s00216-010-3821-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Transfection is a powerful analytical tool enabling study of the function of genes and gene products in cells. The transfection methods are broadly classified into three groups; biological, chemical, and physical. These methods have advanced to make it possible to deliver nucleic acids to specific subcellular regions of cells by use of a precisely controlled laser-microcope system. The combination of point-directed transfection and mRNA transfection is a new way of studying the function of genes and gene products. However, each method has its own advantages and disadvantages so the optimum method depends on experimental design and objective.

引用

页码：3173 / 3178

页数：6

共 36 条

[1] Region-directed phototransfection reveals the functional significance of a dendritically synthesized transcription factor [J].

Barrett, Lindy E. ;

Sul, Jai-Yoon ;

Takano, Hajime ;

Van Bockstaele, Elisabeth J. ;

Haydon, Philip G. ;

Eberwine, James H. .

NATURE METHODS, 2006, 3 (06) :455-460

[2] Delivery of small interfering RNA to mammalian cells in culture by using cationic lipid/polymer-based transfection reagents [J].

Brazas, RM ;

Hagstrom, JE .

RNA INTERFERENCE, 2005, 392 :112-+

[3] Single cell transfection using plasmid decorated AFM probes [J].

Cuerrier, Charles M. ;

Lebel, Rejean ;

Grandbois, Michel .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2007, 355 (03) :632-636

[4]

Davis BR, 2000, CURR OPIN MOL THER, V2, P412

[5] Gene therapy progress and prospects: magnetic nanoparticle-based gene delivery [J].

Dobson, J .

GENE THERAPY, 2006, 13 (04) :283-287

[6] Towards safe, non-viral therapeutic gene expression in humans [J].

Glover, DJ ;

Lipps, HJ ;

Jans, DA .

NATURE REVIEWS GENETICS, 2005, 6 (04) :299-U29

[7] Single-cell electroporation for gene transfer in vivo [J].

Haas, K ;

Sin, WC ;

Javaherian, A ;

Li, Z ;

Cline, HT .

NEURON, 2001, 29 (03) :583-591

[8] Sustained correction of X-linked severe combined immunodeficiency by ex vivo gene therapy [J].

Hacein-Bey-Abina, S ;

Le Deist, F ;

Carlier, F ;

Bouneaud, C ;

Hue, C ;

De Villartay, JP ;

Thrasher, AJ ;

Wulffraat, N ;

Sorensen, R ;

Dupuis-Girod, S ;

Fischer, A ;

Cavazzana-Calvo, M ;

Davies, EG ;

Kuis, W ;

Lundlaan, WHK ;

Leiva, L .

NEW ENGLAND JOURNAL OF MEDICINE, 2002, 346 (16) :1185-1193

[9] A species of small antisense RNA in posttranscriptional gene silencing in plants [J].

Hamilton, AJ ;

Baulcombe, DC .

SCIENCE, 1999, 286 (5441) :950-952

[10] Gene expression using an ultrathin needle enabling accurate displacement and low invasiveness [J].

Han, SW ;

Nakamura, C ;

Obataya, I ;

Nakamura, N ;

Miyake, J .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 332 (03) :633-639

← 1 2 3 4 →