Detection of vaccinia virus DNA, but not infectious virus, in the blood of smallpox vaccine recipients

被引：22
作者：
Cohen, Jeffrey I.
Hohman, Patricia
Preuss, Jeanne C.
Li, Li
Fischer, Steven H.
Fedorko, Daniel P.
机构：
[1] NIH, NIAID, Lab Clin Infect Dis, Med Virol Sect, Bethesda, MD 20892 USA
[2] NIH, Warren G Magnuson Clin Ctr, Bethesda, MD 20892 USA
来源：
VACCINE | 2007年 / 25卷 / 23期
关键词：
smallpox vaccine; vaccinia virus; poxvirus;
D O I：
10.1016/j.vaccine.2007.03.044
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The authors of a recent study [Savona MR, Dela Cruz WP, Jones MS, Thornton JA, Xia D, Hadfield TL, et al. Detection of vaccinia DNA in the blood following smallpox vaccination. JAMA 2006; 295:1898-1900] suggested that the duration of deferral for blood donations by smallpox vaccinees should be extended, based on detection of vaccinia virus DNA in five blood samples by polymerase chain reaction (PCR) and the potential for viremia. We found that 4 of 202 blood specimens (from 3 of 27 smallpox vaccinees) were positive for vaccinia virus DNA by PCR; none were positive for virus by culture. Throat swabs were negative by PCR and culture. Thus, while some blood specimens contained vaccinia virus DNA, infectious virus was not detected. Current guidelines for deferral of blood donation in vaccinees seem appropriate. Published by Elsevier Ltd.
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收藏
页码：4571 / 4574
页数：4
相关论文
共 12 条
  • [1] ANTIBODY RESPONSE TO CUTANEOUS INOCULATION WITH VACCINIA VIRUS - VIREMIA + VIRURIA IN VACCINATED CHILDREN
    BLATTNER, RJ
    HEYS, FM
    AKSU, I
    NORMAN, JO
    [J]. JOURNAL OF PEDIATRICS, 1964, 64 (06) : 839 - +
  • [2] CHO CT, 1973, BACTERIOL REV, V37, P1
  • [3] Lack of vaccinia viremia after smallpox vaccination
    Cummings, JF
    Polhemus, ME
    Hawkes, C
    Klote, M
    Ludwig, GV
    Wortmann, G
    [J]. CLINICAL INFECTIOUS DISEASES, 2004, 38 (03) : 456 - 458
  • [4] VIRUS PARTICLE CONTENT OF SMALLPOX VACCINES
    DUNLAP, RC
    [J]. APPLIED MICROBIOLOGY, 1970, 19 (04) : 689 - &
  • [5] Comparison of methods for detection of vaccinia virus in patient specimens
    Fedorko, DP
    Preuss, JC
    Fahle, GA
    Li, L
    Fischer, SH
    Hohman, P
    Cohen, JI
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (09) : 4602 - 4606
  • [6] Jezek Z., 1988, HUMAN MONKEYPOX, V17
  • [7] KEMPE CH, 1960, PEDIATRICS, V26, P176
  • [8] Absence of oropharyngeal vaccinia virus after vaccinia (smallpox) vaccination
    Klote, MM
    Ludwig, GV
    Ulrich, MP
    Black, LA
    Hack, DC
    Engler, RJM
    Martin, BL
    [J]. ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY, 2005, 94 (06) : 682 - 685
  • [9] Rapid detection and differentiation of human pathogenic orthopox viruses by a fluorescence resonance energy transfer real-time PCR assay
    Panning, M
    Asper, M
    Kramme, S
    Schmitz, H
    Drosten, C
    [J]. CLINICAL CHEMISTRY, 2004, 50 (04) : 702 - 708
  • [10] Detection of vaccinia DNA in the blood following smallpox vaccination
    Savona, MR
    Dela Cruz, WP
    Jones, MS
    Thornton, JA
    Xia, DX
    Hadfield, TL
    Danaher, PJ
    [J]. JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2006, 295 (16): : 1898 - 1900
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