Evaluation of the Fas/FasL signaling pathway in diabetic rat testis

被引：19
作者：
Bayram, S. [1 ]
Kizilay, G. [1 ]
Topcu-Tarladacalisir, Y. [1 ]
机构：
[1] Trakya Univ, Fac Med, Dept Histol & Embryol, TR-22030 Edirne, Turkey
来源：
BIOTECHNIC & HISTOCHEMISTRY | 2016年 / 91卷 / 03期
关键词：
diabetes mellitus; testis; rat; caspase; 8; FasL; immunohistochemistry; Fas; 3; GERM-CELL APOPTOSIS; FAS SYSTEM; UP-REGULATION; INVOLVEMENT; ACTIVATION; EXPRESSION; INCREASES; MECHANISM; ETHANOL; DAMAGE;
D O I：
10.3109/10520295.2015.1129556
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We investigated the role of the Fas/Fas ligand (FasL) signaling pathway in diabetic male infertility. Male rats were divided into two groups: a control group and a streptozotocin induced diabetic group. Thirty days after induction of diabetes, samples of testes were harvested and fixed in 10% formalin for light microscopy. Germ cell apoptosis was determined using the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate in situ nick end-labeling (TUNEL) and immunostaining of caspase 8 and active caspase 3. We also investigated the expressions of Fas and FasL using immunohistochemistry. Streptozotocin-induced diabetes caused severe histopathological damage and increased apoptotic tubule and apoptotic cell indices, caspase 8 and caspase 3 expressions, and Fas and FasL-immunopositive cells in the rat testes. We suggest that the Fas/FasL signaling pathway may play a role in male infertility caused by diabetes.
引用
收藏
页码：204 / 211
页数：8
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