Fluorescent Labeling of Polysaccharides from Masson Pine Pollen and Its Effect on RAW264.7 Macrophages

被引：20
作者：
Sun, Mengmeng [1 ,2 ]
Su, Fangchen [1 ]
Yang, Jinxin [1 ]
Gao, Zheng [1 ]
Geng, Yue [1 ]
机构：
[1] Shandong Normal Univ, Sch Life Sci, Shandong Prov Key Lab Anim Resistance Biol, Jinan 250014, Shandong, Peoples R China
[2] Qilu Univ Technol, Shandong Acad Sci, Coll Bioengn, Jinan 250353, Shandong, Peoples R China
来源：
POLYMERS | 2018年 / 10卷 / 04期
关键词：
polysaccharides; PPM60-Tyr-FITC; RAW264.7; macrophages; ENDOCYTOSIS; PATHWAYS; TLR4; TOLL-LIKE-RECEPTOR-4; RECOGNITION; ACTIVATION; RECEPTOR; CELLS; LPS;
D O I：
10.3390/polym10040372
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
In order to explore the immediate effect of polysaccharides and macrophages, polysaccharides from masson pine pollen (PPM60) were labeled with fluorescein isothiocyanate (FITC) by using a chemical-derived method, and the reactant was named PPM60-Tyr-FITC. Direct interaction of PPM60-Tyr-FITC and RAW264.7 macrophages could be detected by flow cytometer (FCM), and this interaction could be inhibited by Pitstop 2 (clathrin inhibitor) and TAK-242 (Toll-like receptor 4 inhibitor). The results of confocal laser scanning microscopy (CLSM) also revealed that there was a co-localization phenomenon between PPM60-Tyr-FITC and RAW264.7 macrophage receptors, and it could be suppressed by Pitstop 2 and TAK-242. It was confirmed that PPM60 enters into RAW264.7 macrophages mainly through endocytosis, rather than the phagocytosis, and TLR4 played a mediating role.
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页数：11
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