Influence of platelet count, acetylsalicylic acid, von Willebrand's disease, coagulopathies, and haematocrit on results obtained using a platelet function analyser in dogs

The platelet function analyser PFA-100 aspirates blood in vitro from a sample reservoir in disposable test cartridges through a microscopic aperture cut into a biologically active membrane at the end of a capillary. In different cartridges the membrane is coated with collagen and adenosine diphosphate (ADP) or collagen and epinephrine (adrenaline) inducing a platelet plug and closure of the aperture. The closure time and total volume of blood flow through the capillary until closure of its aperture were measured. The correlation between platelet count in samples of thrombocytopenic dogs and results of the collagen/ADP cartridge (closure time: r(s) = -0.579; total volume: r(s) = -0.549) was closer than between platelet count and capillary bleeding time. No significant correlation was observed between platelet count and the results obtained with the collagen/epinephrine cartridge. In addition, a higher sensitivity was obtained for the collagen/ADP cartridge. Injection of acetylsalicylic acid into healthy dogs significantly increased closure time and total volume of both types of cartridges (P < 0.01). Two dogs with von Willebrand's disease had abnormal values. In contrast, coagulopathies did not significantly influence the results of the platelet function analyser (P > 0.05). Despite adequate sensitivity of measurements using the collagen/ADP cartridge to assess quantitative and qualitative platelet disorders in dogs, the influence of haematocrit (P < 0.0001) will limit the clinical application of the analyser. (C) 2002 Elsevier Science Ltd. All rights reserved.