Genetic Basis of Antimicrobial Resistant Gram-Negative Bacteria Isolated From Bloodstream in Brazil

被引：11

作者：

Silveira, Melise Chaves ^{[1
]}

Rocha-de-Souza, Claudio Marcos ^{[1
]}

de Oliveira Santos, Ivson Cassiano ^{[1
]}

Pontes, Leilane da Silva ^{[1
]}

Tavares e Oliveira, Thamirys Rachel ^{[1
]}

Tavares-Teixeira, Camila Bastos ^{[1
]}

Cossatis, Nataly de Almeida ^{[1
]}

Pereira, Natacha Ferreira ^{[1
]}

da Conceicao-Neto, Orlando Carlos ^{[1
]}

da Costa, Bianca Santos ^{[1
]}

Silva Rodrigues, Daiana Cristina ^{[1
]}

Albano, Rodolpho Mattos ^{[2
]}

Barbosa da Silva, Fabricio Alves ^{[3
]}

Marques, Elizabeth Andrade ^{[4
]}

Leao, Robson Souza ^{[4
]}

D'Alincourt Carvalho-Assef, Ana Paula ^{[1
]}

机构：

[1] Inst Oswaldo Cruz FIOCRUZ, Lab Pesquisa Infecc Hosp, Rio De Janeiro, Brazil

[2] Univ Estado Rio de Janeiro UERJ, Dept Bioquim, Inst Biol Roberto Alcantera Gome, Rio De Janeiro, Brazil

[3] Fdn Oswaldo Cruz FIOCRUZ, Programa Comp Cient, Rio De Janeiro, Brazil

[4] Univ Estado Rio de Janeiro UERJ, Dept Microbiol Imunol & Parasitol, Fac Ciencias Med, Rio De Janeiro, Brazil

来源：

FRONTIERS IN MEDICINE | 2021年 / 8卷

关键词：

multidrug-resistance; bloodstream infections; gram-negative bacilli; whole-genome sequencing; Brazil; surveillance; KPC-2-PRODUCING KLEBSIELLA-PNEUMONIAE; GENOME; PLASMIDS; INFECTIONS; ALIGNMENT; SPREAD;

D O I：

10.3389/fmed.2021.635206

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Multidrug-resistant microorganisms are a well-known global problem, and gram-negative bacilli are top-ranking. When these pathogens are associated with bloodstream infections (BSI), outcomes become even worse. Here we applied whole-genome sequencing to access information about clonal distribution, resistance mechanism diversity and other molecular aspects of gram-negative bacilli (GNB) isolated from bloodstream infections in Brazil. It was possible to highlight international high-risk clones circulating in the Brazilian territory, such as CC258 for Klebsiella pneumoniae, ST79 for Acinetobacter baumannii and ST233 for Pseudomonas aeruginosa. Important associations can be made such as a negative correlation between CRISPR-Cas and K. pneumoniae CC258, while the genes bla(TEM), bla(KPC) and bla(CTX-M) are highly associated with this clone. Specific relationships between A. baumannii clones and bla(OXA-51) variants were also observed. All P. aeruginosa ST233 isolates showed the genes bla(VIM) and bla(OXA486). In addition, some trends could be identified, where a new P. aeruginosa MDR clone (ST3079), a novel A. baumannii clonal profile circulating in Brazil (ST848), and important resistance associations in the form of bla(VIM-2) and bla(IMP-56) being found together in one ST233 strain, stand out. Such findings may help to develop approaches to deal with BSI and even other nosocomial infections caused by these important GNB.

引用

页数：9

共 46 条

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