Ky-2, a Histone Deacetylase Inhibitor, Enhances High-Salinity Stress Tolerance in Arabidopsis thaliana

被引：50

作者：

Sako, Kaori ^{[1
]}

Kim, Jong-Myong ^{[1
]}

Matsui, Akihiro ^{[1
]}

Nakamura, Kotaro ^{[2
]}

Tanaka, Maho ^{[1
]}

Kobayashi, Makoto ^{[3
]}

Saito, Kazuki ^{[3
,4
]}

Nishino, Norikazu ^{[5
]}

Kusano, Miyako ^{[3
,6
]}

Taji, Teruaki ^{[2
]}

Yoshida, Minoru ^{[7
]}

Seki, Motoaki ^{[1
,8
,9
]}

机构：

[1] RIKEN, Ctr Sustainable Resource Sci, Plant Genom Network Res Team, Tsurumi Ku, 1-7-22 Suehiro Cho, Yokohama, Kanagawa 2300045, Japan

[2] Tokyo Univ Agr, Fac Appl Biosci, Setagaya Ku, 1-1-1 Sakuragaoka, Tokyo 1568502, Japan

[3] RIKEN, Ctr Sustainable Resource Sci, Metabol Res Grp, Tsurumi Ku, 1-7-22 Suehiro Cho, Yokohama, Kanagawa 2300045, Japan

[4] Chiba Univ, Grad Sch Pharmaceut Sci, Chuo Ku, 1-8-1 Inohana, Chiba 2608522, Japan

[5] Kyushu Inst Technol, Grad Sch Life Sci & Syst Engn, Kitakyushu, Fukuoka 8080196, Japan

[6] Univ Tsukuba, Grad Sch Life & Environm Sci, 1-1-1 Tennodai, Tsukuba, Ibaraki 3058572, Japan

[7] RIKEN, Ctr Sustainable Resource Sci, Chem Genom Res Grp, 2-1 Hirosawa, Wako, Saitama 3510198, Japan

[8] Yokohama City Univ, Kihara Inst Biol Res, Totsuka Ku, 641-12 Maioka Cho, Yokohama, Kanagawa 2440813, Japan

[9] JST, CREST, 4-1-8 Honcho, Kawaguchi, Saitama 3320012, Japan

来源：

PLANT AND CELL PHYSIOLOGY | 2016年 / 57卷 / 04期

基金：

日本学术振兴会; 日本科学技术振兴机构;

关键词：

Chemical biology; HDAC inhibitor; Histone deacetylase; Salinity stress; SALT-TOLERANCE; NA+/H+ ANTIPORTER; PROTEIN-KINASE; UP-REGULATION; GENE; MECHANISMS; RICE; OVEREXPRESSION; ACCUMULATION; RESPONSES;

D O I：

10.1093/pcp/pcv199

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Adaptation to environmental stress requires genome-wide changes in gene expression. Histone modifications are involved in gene regulation, but the role of histone modifications under environmental stress is not well understood. To reveal the relationship between histone modification and environmental stress, we assessed the effects of inhibitors of histone modification enzymes during salinity stress. Treatment with Ky-2, a histone deacetylase inhibitor, enhanced high-salinity stress tolerance in Arabidopsis. We confirmed that Ky-2 possessed inhibition activity towards histone deacetylases by immunoblot analysis. To investigate how Ky-2 improved salt stress tolerance, we performed transcriptome and metabolome analysis. These data showed that the expression of salt-responsive genes and salt stress-related metabolites were increased by Ky-2 treatment under salinity stress. A mutant deficient in AtSOS1 (Arabidopis thaliana SALT OVERLY SENSITIVE 1), which encodes an Na+/H+ antiporter and was among the up-regulated genes, lost the salinity stress tolerance conferred by Ky-2. We confirmed that acetylation of histone H4 at AtSOS1 was increased by Ky-2 treatment. Moreover, Ky-2 treatment decreased the intracellular Na+ accumulation under salinity stress, suggesting that enhancement of SOS1-dependent Na+ efflux contributes to increased high-salinity stress tolerance caused by Ky-2 treatment.

引用

页码：776 / 783

页数：8

共 29 条

[1] Loss of Arabidopsis 5'aEuro"3' Exoribonuclease AtXRN4 Function Enhances Heat Stress Tolerance of Plants Subjected to Severe Heat Stress [J].