Biological pretreatment of corn stover with white-rot fungus for improved enzymatic hydrolysis

被引:146
作者
Saha, Badal C. [1 ]
Qureshi, Nasib [1 ]
Kennedy, Gregory J. [1 ]
Cotta, Michael A.
机构
[1] ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA
关键词
White-rot fungus; Biological pretreatment; Enzymatic hydrolysis; Solid state cultivation; Corn stover; PHANEROCHAETE-CHRYSOSPORIUM; ACID PRETREATMENT; SACCHARIFICATION; BIOMASS; LIGNIN; LIGNOCELLULOSE; FERMENTATION; ETHANOL; STRAW; WOOD;
D O I
10.1016/j.ibiod.2015.12.020
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Biological pretreatment of lignocellulosic biomass by white-rot fungus can represent a low-cost and eco-friendly alternative to harsh physical, chemical or physico-chemical pretreatment methods to facilitate enzymatic hydrolysis. However, fungal pretreatment can cause carbohydrate loss and it is, therefore, necessary to use the appropriate fungal strain-biomass type combination. In this work, 26 white-rot fungal strains were evaluated under solid state cultivation at 74% moisture level at 28 degrees C for 30 days for fermentable sugar production from corn stover after enzymatic hydrolysis of each fungal pretreated corn stover using a cocktail of 3 commercial enzyme (cellulase, beta-glucosidase, hemicellulase) preparations. The best result was obtained with Cyathus stercoreus NRRL-6573 which gave a sugar yield of 394 +/- 13 mg/g of pretreated stover followed by Pycnoporus sanguineus FP-10356-Sp (393 +/- 17 mg/g) and Phlebia brevispora NRRL-13108 (383 +/- 13 mg/g). Published by Elsevier Ltd.
引用
收藏
页码:29 / 35
页数:7
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