A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

被引：51
|
作者：
Huang, Ying [1 ]
Russell, William K. [1 ]
Wan, Wei [2 ]
Pai, Pei-Jing [1 ]
Russell, David H. [1 ]
Liu, Wenshe [1 ]
机构：
[1] Texas A&M Univ, Dept Chem, College Stn, TX 77843 USA
[2] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA
来源：
MOLECULAR BIOSYSTEMS | 2010年 / 6卷 / 04期
关键词：
POSTTRANSLATIONAL MODIFICATIONS; SITE; CODE;
D O I：
10.1039/b920120c
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three N-epsilon-acetyl-L-lysines into one GFP(UV) protein in E. coli.
引用
收藏
页码：683 / 686
页数：4
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