Super-resolution three-dimensional fluorescence and optical diffraction tomography of live cells using structured illumination generated by a digital micromirror device

被引：54
作者：
Shin, Seungwoo [1 ,2 ]
Kim, Doyeon [1 ,2 ,3 ]
Kim, Kyoohyun [1 ,2 ,4 ]
Park, YongKeun [1 ,2 ,3 ]
机构：
[1] Korea Adv Inst Sci & Technol, Dept Phys, 291 Daehak Ro, Daejeon 34141, South Korea
[2] Korea Adv Inst Sci & Technol, KAIST Inst Hlth Sci & Technol, Daejeon 34141, South Korea
[3] Tomocube Inc, 48,Yuseong Daero 1184beon Gil, Daejeon 34051, South Korea
[4] Tech Univ Dresden, Biotechnol Ctr, D-01307 Dresden, Germany
来源：
SCIENTIFIC REPORTS | 2018年 / 8卷
基金：
新加坡国家研究基金会;
关键词：
QUANTITATIVE PHASE MICROSCOPY; RECONSTRUCTION; RAMAN;
D O I：
10.1038/s41598-018-27399-w
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We present a multimodal approach for measuring the three-dimensional (3D) refractive index (RI) and fluorescence distributions of live cells by combining optical diffraction tomography (ODT) and 3D structured illumination microscopy (SIM). A digital micromirror device is utilized to generate structured illumination patterns for both ODT and SIM, which enables fast and stable measurements. To verify its feasibility and applicability, the proposed method is used to measure the 3D RI distribution and 3D fluorescence image of various samples, including a cluster of fluorescent beads, and the time-lapse 3D RI dynamics of fluorescent beads inside a HeLa cell, from which the trajectory of the beads in the HeLa cell is analyzed using spatiotemporal correlations.
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页数：8
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