Super-resolution three-dimensional fluorescence and optical diffraction tomography of live cells using structured illumination generated by a digital micromirror device

被引：54
作者：
Shin, Seungwoo [1 ,2 ]
Kim, Doyeon [1 ,2 ,3 ]
Kim, Kyoohyun [1 ,2 ,4 ]
Park, YongKeun [1 ,2 ,3 ]
机构：
[1] Korea Adv Inst Sci & Technol, Dept Phys, 291 Daehak Ro, Daejeon 34141, South Korea
[2] Korea Adv Inst Sci & Technol, KAIST Inst Hlth Sci & Technol, Daejeon 34141, South Korea
[3] Tomocube Inc, 48,Yuseong Daero 1184beon Gil, Daejeon 34051, South Korea
[4] Tech Univ Dresden, Biotechnol Ctr, D-01307 Dresden, Germany
来源：
SCIENTIFIC REPORTS | 2018年 / 8卷
基金：
新加坡国家研究基金会;
关键词：
QUANTITATIVE PHASE MICROSCOPY; RECONSTRUCTION; RAMAN;
D O I：
10.1038/s41598-018-27399-w
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We present a multimodal approach for measuring the three-dimensional (3D) refractive index (RI) and fluorescence distributions of live cells by combining optical diffraction tomography (ODT) and 3D structured illumination microscopy (SIM). A digital micromirror device is utilized to generate structured illumination patterns for both ODT and SIM, which enables fast and stable measurements. To verify its feasibility and applicability, the proposed method is used to measure the 3D RI distribution and 3D fluorescence image of various samples, including a cluster of fluorescent beads, and the time-lapse 3D RI dynamics of fluorescent beads inside a HeLa cell, from which the trajectory of the beads in the HeLa cell is analyzed using spatiotemporal correlations.
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页数：8
相关论文
共 54 条
  • [1] AGARD DA, 1989, METHOD CELL BIOL, V30, P353
  • [2] Investigating fibroblast cells under "safe" and "injurious" blue-light exposure by holographic microscopy
    Calabuig, Alejandro
    Mugnano, Martina
    Miccio, Lisa
    Grilli, Simonetta
    Ferraro, Pietro
    [J]. JOURNAL OF BIOPHOTONICS, 2017, 10 (6-7) : 919 - 927
  • [3] Cho S., 2013, SCI REPORTS, V3
  • [4] Structured illumination microscopy for dual-modality 3D sub-diffraction resolution fluorescence and refractive-index reconstruction
    Chowdhury, Shwetadwip
    Eldridge, Will J.
    Wax, Adam
    Izatt, Joseph A.
    [J]. BIOMEDICAL OPTICS EXPRESS, 2017, 8 (12): : 5776 - 5793
  • [5] Structured illumination multimodal 3D-resolved quantitative phase and fluorescence sub-diffraction microscopy
    Chowdhury, Shwetadwip
    Eldridge, Will J.
    Wax, Adam
    Izatt, Joseph A.
    [J]. BIOMEDICAL OPTICS EXPRESS, 2017, 8 (05): : 2496 - 2518
  • [6] Multiple phases of chondrocyte enlargement underlie differences in skeletal proportions
    Cooper, Kimberly L.
    Oh, Seungeun
    Sung, Yongjin
    Dasari, Ramachandra R.
    Kirschner, Marc W.
    Tabin, Clifford J.
    [J]. NATURE, 2013, 495 (7441) : 375 - 378
  • [7] Cotte Y, 2013, NAT PHOTONICS, V7, P113, DOI [10.1038/nphoton.2012.329, 10.1038/NPHOTON.2012.329]
  • [8] DMD-based LED-illumination Super-resolution and optical sectioning microscopy
    Dan, Dan
    Lei, Ming
    Yao, Baoli
    Wang, Wen
    Winterhalder, Martin
    Zumbusch, Andreas
    Qi, Yujiao
    Xia, Liang
    Yan, Shaohui
    Yang, Yanlong
    Gao, Peng
    Ye, Tong
    Zhao, Wei
    [J]. SCIENTIFIC REPORTS, 2013, 3
  • [9] Integral refractive index imaging of flowing cell nuclei using quantitative phase microscopy combined with fluorescence microscopy
    Dardikman, Gilt
    Nygate, Yoav N.
    Barnea, Itay
    Turko, Nir A.
    Singh, Gyanendra
    Javidi, Barham
    Shaked, Natan T.
    [J]. BIOMEDICAL OPTICS EXPRESS, 2018, 9 (03): : 1177 - 1189
  • [10] Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI)
    Dertinger, T.
    Colyer, R.
    Iyer, G.
    Weiss, S.
    Enderlein, J.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (52) : 22287 - 22292
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