NF-κB promotes epithelial-mesenchynmal transition, migration and invasion of pancreatic carcinoma cells

被引：197
作者：
Maier, Harald J. [1 ]
Schmidt-Strassburger, Uta [1 ]
Huber, Margit A. [2 ,3 ]
Wiedemann, Eva M. [3 ]
Beug, Hartmut [3 ]
Wirth, Thomas [1 ]
机构：
[1] Univ Ulm, Inst Physiol Chem, D-89081 Ulm, Germany
[2] Univ Ulm, Dept Dermatol & Allerg Dis, D-89081 Ulm, Germany
[3] Res Inst Mol Pathol, A-1030 Vienna, Austria
来源：
CANCER LETTERS | 2010年 / 295卷 / 02期
关键词：
NF-kappa B; Epithelial-mesenchymal transition; Pancreatic adenocarcinoma; Tumour progression; Cell migration; Cell invasion; GROWTH-FACTOR; SNAIL TRANSCRIPTION; GENE-EXPRESSION; TUMOR-GROWTH; ACTIVATION; CANCER; ALPHA; BETA; INFLAMMATION; ADENOCARCINOMA;
D O I：
10.1016/j.canlet.2010.03.003
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The transcription factor NF-kappa B is constitutively active in pancreatic adenocarcinoma. Here we explore the contribution of NF-kappa B to the malignant phenotype of pancreatic cancer cells in addition to its anti-apoptotic role. Block of NF-kappa B signalling by non-destructible ham rendered cells resistant to TGF-beta-induced epithelial-mesenchymal transition (EMT). In contrast, NF-kappa B activation by TNF-alpha or expression of constitutively active IKK2 induced an EMT-phenotype with up-regulation of vimentin and ZEB1, and down-regulation of E-cadherin. EMT could also be induced in cells with defective TGF-beta signalling. Functional assays demonstrated reduced or strongly enhanced migration and invasion upon NF-kappa B inhibition or activation, respectively. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：214 / 228
页数：15
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