Rapid determination of the synthetic activity of lipases/esterases via transesterification and esterification zymography

被引：24

作者：

Duarte, Jaqueline G. ^{[1
]}

Leone-Ignacio, Kassia ^{[1
]}

da Silva, Jose Andre C. ^{[2
]}

Fernandez-Lafuente, Roberto ^{[3
]}

Freire, Denise M. G. ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Dept Biochem, Inst Chem, Ctr Tecnol,Ilha Fundao, Bl A,Sl 549, BR-21949900 Rio De Janeiro, Brazil

[2] Cenpes, Ctr Pesquisas & Desenvolvimento Leopoldo Amer Mig, Rio De Janeiro, Brazil

[3] ICP CSIC, Dept Biocatalisis, C Marie Curie 2,Campus UAM CSIC, Madrid 28049, Spain

来源：

FUEL | 2016年 / 177卷

关键词：

Transesterification reactions; Zymography; Biodiesel; Lipase; Biolubricants; BIODIESEL PRODUCTION; IMMOBILIZED LIPASE; OIL; OPTIMIZATION; PURIFICATION; HYDROLYSIS; ACID;

D O I：

10.1016/j.fuel.2016.02.079

中图分类号：

TE [石油、天然气工业]; TK [能源与动力工程];

学科分类号：

0807 ; 0820 ;

摘要：

A new simple and extremely versatile zymography method based on transesterification and esterification reactions was developed in this work. The method consists in building a transesterification or esterification reaction medium according to the goal of the research. Since commercial enzymes and crude extracts commonly consist in pools of proteins, this methodology provides a means to determine which protein are responsible for the enzymatic activity desired. The protocol may be potentially used as a high-throughput screening of lipases or esterases that catalyzes the synthesis reaction for biodiesel and biolubricants production. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：123 / 129

页数：7

共 23 条

[11] Advances in synthesis of biodiesel via enzyme catalysis: Novel and sustainable approaches [J].

Guldhe, Abhishek ;

Singh, Bhaskar ;

Mutanda, Taurai ;

Perrnaul, Kugen ;

Bux, Faizal .

RENEWABLE & SUSTAINABLE ENERGY REVIEWS, 2015, 41 :1447-1464

[12] Purification and characterisation of an ester hydrolase from a strain of Arthrobacter species:: Its application in asymmetrisation of 2-benzyl-1,3-propanediol acylates [J].

Johri, S ;

Verma, V ;

Parshad, R ;

Koul, S ;

Taneja, SC ;

Qazi, GN .

BIOORGANIC & MEDICINAL CHEMISTRY, 2001, 9 (02) :269-273

[13] Kinetics and optimization of lipase-catalyzed synthesis of rose fragrance 2-phenylethyl acetate through transesterification [J].

Kuo, Chia-Hung ;

Chen, Guan-Jie ;

Chen, Chih-I ;

Liu, Yung-Chuan ;

Shieh, Chwen-Jen .

PROCESS BIOCHEMISTRY, 2014, 49 (03) :437-444

[14] Synthesis activity-based zymography for detection of lipases and esterases [J].

Kwon, Min-A ;

Kim, Hyun Suk ;

Hahm, Dae-Hyun ;

Song, Jae Kwang .

BIOTECHNOLOGY LETTERS, 2011, 33 (04) :741-746

[15] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[16]

Laemmli UK, 1970, NATURE, V227, P15

[17] An optimization study on transesterification catalyzed by the activated carbide slag through the response surface methodology [J].

Liu, Mengqi ;

Niu, Shengli ;

Lu, Chunmei ;

Cheng, Shiqing .

ENERGY CONVERSION AND MANAGEMENT, 2015, 92 :498-506

[18] Lipase catalyzed transesterification of castor oil by straight chain higher alcohols [J].

Malhotra, Deepika ;

Mukherjee, Joyeeta ;

Gupta, Munishwar N. .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2015, 119 (03) :280-283

[19] ZYMOGRAM OF PROTEASES MADE WITH DEVELOPED FILM FROM NONDENATURING POLYACRYLAMIDE GELS AFTER ELECTROPHORESIS [J].

PAECH, C ;

CHRISTIANSON, T ;

MAURER, KH .

ANALYTICAL BIOCHEMISTRY, 1993, 208 (02) :249-254

[20] Purification strategies for microbial lipases [J].

Saxena, RK ;

Sheoran, A ;

Giri, B ;

Davidson, WS .

JOURNAL OF MICROBIOLOGICAL METHODS, 2003, 52 (01) :1-18

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