Rapid determination of the synthetic activity of lipases/esterases via transesterification and esterification zymography

被引：24

作者：

Duarte, Jaqueline G. ^{[1
]}

Leone-Ignacio, Kassia ^{[1
]}

da Silva, Jose Andre C. ^{[2
]}

Fernandez-Lafuente, Roberto ^{[3
]}

Freire, Denise M. G. ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Dept Biochem, Inst Chem, Ctr Tecnol,Ilha Fundao, Bl A,Sl 549, BR-21949900 Rio De Janeiro, Brazil

[2] Cenpes, Ctr Pesquisas & Desenvolvimento Leopoldo Amer Mig, Rio De Janeiro, Brazil

[3] ICP CSIC, Dept Biocatalisis, C Marie Curie 2,Campus UAM CSIC, Madrid 28049, Spain

来源：

FUEL | 2016年 / 177卷

关键词：

Transesterification reactions; Zymography; Biodiesel; Lipase; Biolubricants; BIODIESEL PRODUCTION; IMMOBILIZED LIPASE; OIL; OPTIMIZATION; PURIFICATION; HYDROLYSIS; ACID;

D O I：

10.1016/j.fuel.2016.02.079

中图分类号：

TE [石油、天然气工业]; TK [能源与动力工程];

学科分类号：

0807 ; 0820 ;

摘要：

A new simple and extremely versatile zymography method based on transesterification and esterification reactions was developed in this work. The method consists in building a transesterification or esterification reaction medium according to the goal of the research. Since commercial enzymes and crude extracts commonly consist in pools of proteins, this methodology provides a means to determine which protein are responsible for the enzymatic activity desired. The protocol may be potentially used as a high-throughput screening of lipases or esterases that catalyzes the synthesis reaction for biodiesel and biolubricants production. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：123 / 129

页数：7

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