Sulfated Chitosan Oligosaccharides Suppress LPS-Induced NO Production via JNK and NF-κB Inactivation

被引：44

作者：

Kim, Jung-Hyun ^{[1
]}

Kim, Yon-Suk ^{[1
]}

Hwang, Jin-Woo ^{[1
]}

Han, Young-Ki ^{[1
]}

Lee, Jung-Suck ^{[2
]}

Kim, Se-Kwon ^{[3
]}

Jeon, You-Jin ^{[4
]}

Moon, Sang-Ho ^{[5
]}

Jeon, Byong-Tae ^{[5
]}

Bahk, Young Yil ^{[1
]}

Park, Pyo-Jam ^{[1
,5
]}

机构：

[1] Konkuk Univ, Dept Biotechnol, Chungju 380701, South Korea

[2] Jeju Natl Univ, Ind Acad Cooperat Fdn, Cheju 690756, South Korea

[3] Pukyong Natl Univ, Specialized Grad Sch Technol Convergence, Dept Marine Bioconvergence Sci, Pusan 608737, South Korea

[4] Jeju Natl Univ, Sch Marine Biomed Sci, Cheju 690756, South Korea

[5] Konkuk Univ, Korea Nokyong Res Ctr, Chungju 380701, South Korea

来源：

MOLECULES | 2014年 / 19卷 / 11期

关键词：

sulfated chitosan oligosaccharides; RAW264.7; cells; MAPKs pathways; anti-inflammatory; NITRIC-OXIDE SYNTHASE; RAW; 264.7; MACROPHAGES; SIGNALING PATHWAYS; ANTIOXIDANT ACTIVITY; SENTICOSUS EXTRACT; CELLS; EXPRESSION; CHITOOLIGOSACCHARIDES; INHIBITION; ACTIVATION;

D O I：

10.3390/molecules191118232

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Various biological effects have been reported for sulfated chitosan oligosaccharides, but the molecular mechanisms of action of their anti-inflammatory effects are still unknown. This study aimed to evaluate the anti-inflammatory effects of sulfated chitosan oligosaccharides and to elucidate the possible mechanisms of action. The results showed that pretreated low molecular weight sulfated chitosan oligosaccharides inhibited the production of nitric oxide (NO) and inflammatory cytokines such as IL-6 and TNF-alpha in lipopolysaccharide (LPS)-activated RAW264.7 cells. The sulfated chitosan oligosaccharides also suppressed inducible nitric oxide synthase (iNOS), phosphorylation of JNK and translocation of p65, a subunit of NF-kappa B, into the nucleus by inhibiting degradation of I kappa B-alpha. Our investigation suggests sulfated chitosan oligosaccharides inhibit IL-6/TNF-alpha in LPS-induced macrophages, regulated by mitogen-activated protein kinases (MAPKs) pathways dependent on NF-kappa B activation.

引用

页码：18232 / 18247

页数：16

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