Structural Characterization and Chain Conformation of Water-Soluble β-Glucan from Wild Cordyceps sinensis

被引：28

作者：

Wang, Junqiao ^{[1
]}

Chen, Shuping ^{[1
]}

Nie, Shaoping ^{[1
]}

Cui, Steve W. ^{[1
,2
]}

Wang, Qi ^{[3
]}

Phillips, Aled O. ^{[3
]}

Phillips, Glyn O. ^{[4
]}

Xie, Mingyong ^{[1
]}

机构：

[1] Nanchang Univ, China Canada Joint Lab Food Sci & Technol Nanchan, State Key Lab Food Sci & Technol, 235 Nanjing East Rd, Nanchang 330047, Jiangxi, Peoples R China

[2] Agr & Agri Food Canada, Guelph Res & Dev Ctr, 93 Stone Rd West, Guelph, ON N1G 5C9, Canada

[3] Univ Cardiff, Sch Med, Cardiff CF10 3AT, S Glam, Wales

[4] Glyndwr Univ, Phillips Hydrocolloids Res Ctr, Wrexham LL11 2AW, Wales

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2019年 / 67卷 / 45期

基金：

中国国家自然科学基金;

关键词：

Cordyceps sinensis; beta-D-glucan; structure; conformation; MOLECULAR-WEIGHT; ANTITUMOR-ACTIVITY; LIGHT-SCATTERING; BODIES;

D O I：

10.1021/acs.jafc.9b05340

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Water-soluble beta-D-glucan was obtained from wild Cordyceps sinensis by alkali solution and ethanol precipitation. The structure characteristics were determined using high-performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD), methylation combined with gas chromatography-mass spectrometry, and one-/two-dimensional nuclear magnetic resonance spectroscopy. Results showed that beta-D-glucan had a structure of every seven (1 -> 3)-beta-D-Glcp backbone residues with two (1 -> 6)-beta-D-Glcp branches. Additionally, conformation properties in different solvents were investigated by static light scattering, dynamic light scattering, and HPSEC with multiple detectors. It was found that beta-D-glucan in 0.5 M NaOH had a narrow unimodal distribution of hydrodynamic radius displaying a spherical coil conformation, whereas it formed severe aggregation in dimethyl sulfoxide. In 0.1 M NaNO3, beta-D-glucan mainly existed as a rod-like conformation corresponding to a helical structure together with small aggregates (10%). This work added more information to the understanding of C. sinensis polysaccharides.

引用

页码：12520 / 12527

页数：8

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