Voltammetric detection of damage to DNA caused by nitro derivatives of fluorene using an electrochemical DNA biosensor

被引：53

作者：

Vyskocil, Vlastimil ^{[1
]}

Labuda, Jan ^{[2
]}

Barek, Jiri ^{[1
]}

机构：

[1] Charles Univ Prague, Fac Sci, Dept Analyt Chem, UNESCO Lab Environm Electrochem, Prague 12843 2, Czech Republic

[2] Slovak Univ Technol Bratislava, Fac Chem & Food Technol, Inst Analyt Chem, Bratislava 81237, Slovakia

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2010年 / 397卷 / 01期

关键词：

2-Nitrofluorene; 2,7-Dinitrofluorene; DNA biosensor; Screen printed carbon paste electrode; Electrochemical detection; DNA damage; POLYCYCLIC AROMATIC-HYDROCARBONS; COMPOSITE ELECTRODE; SILVER; 2-NITROFLUORENE; METABOLISM; 1,10-PHENANTHROLINE; POLAROGRAPHY; FLAVONOIDS; COMPLEXES; CARBON;

D O I：

10.1007/s00216-010-3517-y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An electrochemical DNA biosensor based on the screen printed carbon paste electrode (SPCPE) with an immobilized layer of calf thymus double-stranded DNA has been used for in vitro investigation of the interaction between genotoxic nitro derivatives of fluorene (namely 2-nitrofluorene and 2,7-dinitrofluorene) and DNA. Two types of DNA damage have been detected at the DNA/SPCPE biosensor: first, that caused by direct association of the nitrofluorenes, for which an intercalation association has been found using the known DNA intercalators [Cu(phen)(2)](2+) and [Co(phen)(3)](3+) as competing agents, and, second, that caused by short-lived radicals generated by electrochemical reduction of the nitro group (observable under specific conditions only).

引用

页码：233 / 241

页数：9

共 64 条

[1] Detection of the damage caused to DNA by niclosamide using an electrochemical DNA-biosensor [J].