A sensitive and selective resonance Rayleigh scattering method for quick detection of avidin using affinity labeling Au nanoparticles

被引：9

作者：

Wang, Qi ^{[1
]}

Huang, Xi ^{[1
]}

Fu, Xuan ^{[1
]}

Deng, Huan ^{[1
]}

Ma, Meihu ^{[1
]}

Cai, Zhaoxia ^{[1
]}

机构：

[1] Huazhong Agr Univ, Natl Res & Dev Ctr Egg Proc, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2016年 / 162卷

基金：

中国国家自然科学基金;

关键词：

Avidin; Resonance Rayleigh scattering; Au nanoparticle; Biotin; Egg products; SODIUM DODECYLBENZENE SULFONATE; LIGHT-SCATTERING; GOLD NANOPARTICLES; SPECTRAL PROBE; QUANTUM DOTS; BIOTIN; ASSAY; PROTEINS; STREPTAVIDIN; ION;

D O I：

10.1016/j.saa.2016.02.047

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Avidin is a glycoprotein with antinutritional property, which should be limited in daily food. We developed an affinity biosensor system based on resonance Rayleigh scattering (RRS) and using affinity biotin labeling Au nanoparticles (AuNPs). This method was selective and sensitive for quick avidin detection due to the avidin-biotin affinitive interaction. Under optimal conditions, RRS intensity of biotin-AuNPs increase linearly with an increasing concentration of avidin from 5 to 160 ng/mL. The lower limit of detection was 0.59 ng/mL. This rapid and selective avidin detection method was used in synthetic samples and egg products with recoveries of between 102.97 and 107.92%, thereby demonstrating the feasible and practical application of this assay. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：75 / 80

页数：6

共 39 条

[1] Gold nanoparticle-based quantitative electrochemical detection of amplified human cytomegalovirus DNA using disposable microband electrodes [J].