TNF-α and IL-1α induce apoptosis in subconfluent rat mesangial cells.: Evidence for the involvement of hydrogen peroxide and lipid peroxidation as second messengers

被引：61

作者：

Böhler, T ^{[1
]}

Waiser, J ^{[1
]}

Hepburn, H ^{[1
]}

Gaedeke, J ^{[1
]}

Lehmann, C ^{[1
]}

Hambach, P ^{[1
]}

Budde, K ^{[1
]}

Neumayer, HH ^{[1
]}

机构：

[1] Humboldt Univ, Dept Internal Med Nephrol, Berlin, Germany

来源：

CYTOKINE | 2000年 / 12卷 / 07期

关键词：

apoptosis; hydrogen peroxide; IL-1; alpha; lipid peroxidation; mesangial cells; TNF-alpha;

D O I：

10.1006/cyto.1999.0633

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Apoptosis of mesangial cells (MC) plays a role in glomerulonephritis (GN). In this study we investigated cytokine-induced apoptosis of cultured rat MC by morphological and biochemical features. TNF-alpha and IL-1 alpha induced apoptosis in rat MC in a time- and concentration-dependent fashion. RT-PCR experiments revealed that MC express the TNF-receptor 1 (p60) gene constitutively, TNF-alpha as well as IL-1 alpha stimulated the production of reactive oxygen species (ROS) and induced lipid peroxidation, Coincubation with catalase inhibited TNF-alpha and IL-1 alpha induced apoptosis as well as lipid peroxidation. TNF-alpha, but not IL-1 alpha increased the expression of c-jun, These results provide evidence that TNF-alpha and IL-1 alpha induce apoptosis in rat MC with hydrogen peroxide and lipid peroxidation as second messengers. Increased c-jun expression may be a downstream intracellular signal of TNF-alpha-, but not IL-1 alpha-induced apoptosis. (C) 2000 Academic Press.

引用

页码：986 / 991

页数：6

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