Influence of glycerol on production and structural-physical properties of cellulose from Acetobacter sp V6 cultured in shake flasks

被引:76
作者
Jung, Ho-Il [1 ]
Jeong, Jin-Ha [1 ]
Lee, O-Mi [2 ]
Park, Geun-Tae [3 ]
Kim, Keun-Ki [1 ]
Park, Hyean-Cheal [1 ]
Lee, Sang-Mong [1 ]
Kim, Young-Gyun [1 ]
Son, Hong-Joo [1 ]
机构
[1] Pusan Natl Univ, Dept Life Sci & Environm Biochem, Miryang 627706, South Korea
[2] Korea Atom Energy Res Inst, Radiat Technol Inst, Jeongeup 580185, South Korea
[3] Pusan Natl Univ, Res & Univ Ind Cooperat, Pusan 609735, South Korea
关键词
Acetobacter sp; Cellulose; Glycerol; Low-cost substrate; DIFFERENT CARBON-SOURCES; BACTERIAL CELLULOSE; GLUCONACETOBACTER-XYLINUS; FERMENTATION; GLUCOSE;
D O I
10.1016/j.biortech.2009.12.111
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Cost-effective production of bacterial cellulose (BC) by Acetobacter sp. V6 was investigated in shake culture using glycerol as carbon source and its structural and physical properties were determined. in medium containing 3% (w/v) glycerol, BC production was 4.98 +/- 0.03 g/l after 7 days. This value was 3.8-fold higher than the yield in the glucose medium. FT-IR spectra revealed that all the BC samples were highly crystalline and were cellulose type I. The crystallinity index value of the BC produced was 9% higher in the glycerol medium than in the glucose medium. Scanning electron micrographs showed that BC from the glycerol medium was more compact than that from the glucose medium. Water-holding capacity and viscosity of BC from the glycerol medium had 61.3% and 22.4% lower values than those from the glucose medium. These results suggest that glycerol could be a potential low-cost substrate for BC production by Acetobacter sp. V6, leading to the reduction in the production cost. (c) 2009 Elsevier Ltd. All rights reserved.
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页码:3602 / 3608
页数:7
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