Analysis of soil microbial communities based on amplicon sequencing of marker genes

被引：186

作者：

Schoeler, Anne ^{[1
]}

Jacquiod, Samuel ^{[2
]}

Vestergaard, Gisle ^{[1
]}

Schulz, Stefanie ^{[1
]}

Schloter, Michael ^{[1
,3
]}

机构：

[1] German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Res Unit Comparat Microbiome Anal, D-85764 Neuherberg, Germany

[2] INRA Dijon, Agroecol UMR1347, Dijon, France

[3] Tech Univ Munich, Chair Soil Sci, Munich, Germany

来源：

BIOLOGY AND FERTILITY OF SOILS | 2017年 / 53卷 / 05期

关键词：

Next-generation sequencing; Amplicon sequencing; 16S rRNA; Sequencing depth; Bioinformatics; PCR PRIMERS; MAIZE LINES; DIVERSITY; GENERATION;

D O I：

10.1007/s00374-017-1205-1

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

The use of cultivation independent methods has revolutionized soil biology in the last decades. Most popular approaches are based on directly extracted DNA from soil and subsequent analysis of PCR-amplified marker genes by next-generation sequencing. While these high-throughput methods offer novel possibilities over cultivation-based approaches, several key points need to be considered to minimize potential biases during library preparation and downstream bioinformatic analysis. This opinion paper highlights crucial steps that should be considered for accurate analysis and data interpretation.

引用

页码：485 / 489

页数：5

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